CAS: 42935-24-0; P-Aminophenyl β-D-Cellobioside

相似化合物

3482-57-3 17691-02-0 150412-80-9

上下游产品

CAS号3482-57-3 4-硝基苯基-β-D-纤维二糖苷 | CAS号100-02-7 对硝基苯酚 | CAS号69948-03-4 对硝基苯基β-D-芹菜素七乙酸酯

📜4-硝基苯基-β-D-纤维二糖苷置于palladium On Activated Charcoal 氢气体系中,化学反应生成 4-氨基苯基-β-D-吡喃纤维二糖苷
参考文献:P-Aminophenyl β-Cellobioside As An Affinity Ligand For Exo-Type Cellulases
标题:P-Aminophenyl β-Cellobioside As An Affinity Ligand For Exo-Type Cellulases
摘要:P-Aminophenyl Beta-Cellobioside (Papc) Is Shown To Be An Effective Affinity Ligand For The Chromatographic Fractionation Of Cellobiohydrolases (Cbhs). A Crude Cellulase Preparation From The Filamentous Fungus Trichoderma Reesei Served As A Representative Source Of Enzymes Far This Study. Prior To Chromatography,Papc Was Tethered Via Its Amino Functional Group To N-Hydroxysuccinimide-Activated Agarose. The Resulting Affinity Matrix Specifically Retained The Cbh Component Of Relatively Complex Cellulase Mixtures. The Purity Of The Resulting Cbh Preparations,Based On Measured Specific Activities,Was Comparable To That Of Corresponding Enzyme Preparations Obtained Using More Traditional Thioglycoside-Based Affinity Ligands. The Application Of Papc As An Affinity Ligand Illustrates That The Tethered Ligand Associates With The T. Reesei Cbhs In A Catalytically Nonproductive Mode. In Contrast,The Free Ligand Is Readily Hydrolyzed By T. Reesei Cbh I. (C) 1998 Elsevier Science Ltd. All Rights Reserved.
DOI:10.1016/s0008-6215(98)00294-8