CAS: 3252-36-6; 5-Bromo-4-Chloro-3-Indolyl Acetate

该化合物是一种合成有机化合物,通常用作生物化学实验中的基质,特别是用于检测-galactosidase活性.该化合物具有一种单极结构,由双环化合物组成,由连接到发热圈的苯环组成,代之以溴和氯原子,有助于其在酶反应中的再活动性和特殊性.乙酸酯组增强溶性性和稳定性,使之适合各种实验室应用.在物理特性方面,它一般在室内温度上是固态,在有机溶剂中可能表现出中等溶性.卤素子的出现可以影响其电子特性,使其在分子生物学和生物化学化学学中成为通过色度变化对酶活动进行可视化的有用工具.在处理该化合物时,应采取安全防范措施,因为如果摄入或吸入,可能会对健康造成危害.

结构式图片

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CAS号3030-06-6 1-乙酰基-5-溴-4-氯-3-吲哚乙酯 | CAS号29245-44-1 Vat Blue 2

合成工艺路线路线简述

    1-乙酰基-5-溴-4-氯吲哚-3-乙酸酯置于sodium Hydroxide,水体系中,化学反应生成 N-乙酰基-5-溴-3-羟基吲哚
    参考文献:Holt; Sadler,Proceedings Of The Royal Society Of London. Series B,Biological Sciences,1958,Vol. 148,P. 481,491,493
    标题:Holt; Sadler,Proceedings Of The Royal Society Of London. Series B,Biological Sciences,1958,Vol. 148,P. 481,491,493

    海关参考信息

    专利信息


    专利号:US-2023317208-A1
    优先权日:2022-03-31
    标 题 :Biopolymer synthesis
    发明人:VLADAR HAROLD P; AGOZZINO LUCA
    权利人:RIBBON BIOLABS GMBH
    摘要:The invention provides methods for synthesizing large biopolymers such as genome-scale polynucleotide molecules. Where genome-scale polynucleotides are to be made by attaching together a number of oligos, assembly of the desired polynucleotide from the oligos is represented as a tree, in which the branches represent nucleotide sequences and the nodes represent attachments between pairs of oligos. Numerous assembly trees are each generated and scored in silico according to how successful that tree will be in directing assembly of the desired polynucleotide, given biochemical properties of the oligos and proposed ligations. Systems and methods of the invention select a suitable tree and operate liquid handling systems to perform operations as shown by the selected tree to make the desired polynucleotide.

    专利号:US-7371571-B2
    优先权日:2002-12-17
    标题:Recombinant mycobacteria overexpressing D-alanine ligase gene and uses therefore
    发明人:BARLETTA RAUL G; FENG ZHENGYU
    权利人:UNIV NEBRASKA
    摘要:Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of D -alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.
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    合成参考文献


    参考文献:10.1016/s1040-8428(85)80003-2
    摘要:Thiel E. Cell surface markers in leukemia: Biological and clinical correlations. Critical Reviews in Oncology/Hematology. 1985 Jan;2(3):209–60. doi: 10.1016/s1040-8428(85)80003-2.
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