CAS: 80890-47-7; Concanamycin A

专利信息

专利号:WO-9958147-A1
优先权日:1998-05-08
标 题 :APOPTOSIS OF NAIVE HUMAN NK CELLS BY CROSSLINKING OF THEIR FcγRIIIa MOLECULES WIHT A RAT IgG2b (LO-CD2a/BTI-322) OR ITS IgG1 HUMANIZED MONOCLONAL ANTIBODY
发明人：BAZIN HERVE; LATINNE DOMINIQUE
权利人:UNIV CATHOLIQUE LOUVAIN
摘要:Although the mechanism of induction of apoptosis through antibody dependent cellular cytotoxicity (ADCC) mediated by NK cells is well understood, little is known about the fate of the reactive NK cells. Nevertheless, it has been shown that NK cells previously activated by IL-2, but not naive NK cells, died by apoptosis after FcγRIIIa crosslinking, or after engagement in cytolytic functions. It is demonstrated that apoptosis of naive NK cells is also observed after stimulation with a rat IgG2b anti CD2 mAb (LO-CD2a/BTI-322) or anti HLAI (LO-HLA-1)mAb. The NK apoptosis is rapid (within minutes), Fas-ligand and mRNA synthesis independent and does not require a cell contact. The intracellular mechanism of NK cell apoptosis is calcium, PKC and PLA2 dependent but calcineurin and P13 kinase independent. We suggest that NK cell apoptosis results from the crosslinking on the same cell surface of CD2 or HLA-I molecules and FcγRIIIa that exhibits a high affinity for the rat IgG2b isotype. The clinical application of these experiments are discussed.

专利号:US-6692743-B1
优先权日:1998-05-08
标 题:Apoptosis of naive human NK cells by crosslinking of their FcγRIIIa molecules with a rat IgG2b (LO-CD2a/BTI-322) or its IgG1 humanized monoclonal antibody
发明人：BAZIN HERVE; LATINNE DOMINIQUE
权利人:UNIV CATHOLIQUE LOUVAIN
摘要:Although the mechanism of induction of apoptosis through antibody dependent cellular cytotoxicity (ADCC) mediated by NK cells is well understood, little is known about the fate of the reactive NK cells. Nevertheless, it has been shown that NK cells previously activated by IL-2, but not naive NK cells, died by apoptosis after FcγRIIIa crosslinking, or after engagement in cytolytic functions. It is demonstrated that apoptosis of naive NK cells is also observed after stimulation with a rat IgG2 b anti CD2 mAb (LO-CD2 a /BTI-322) or anti HLAI (LO-HLA-1)mAb. The NK apoptosis is rapid (within minutes), Fas-ligand and mRNA synthesis independent and does not require a cell contact. The intracellular mechanism of NK cell apoptosis is calcium, PKC and PLA2 dependent but calcineurin and P13 kinase independent. We suggest that NK cell apoptosis results from the crosslinking on the same cell surface of CD2 or HLA-I molecules and FcγRIIIa that exhibits a high affinity for the rat IgG2 b isotype. The clinical application of these experiments are discussed.

专利号:EP-0635577-B1
优先权日:1993-07-23
标 题 :Screen for inhibitors of melanin biosynthesis
发明人：BRINDLE FRANCES HARRIET ELIZAB; FROELIGER EUNICE HARRIET
权利人:BASF AG
摘要:A method for the identification of agents that inhibit melanin biosynthesis involves the incubation of test samples in cultures of a fungus that produces melanin. Observation of altered pigmentation in the culture or culture area containing test sample indicates inhibition of melanin biosynthesis. Preferred cultures for the screen contain a fungus that pigments in the light or the dark such as Cladosporium cucumerinum. Preferred embodiments employ test samples on disks or in wells in solidified cultures and a known melanin synthesis inhibitor as a positive control which is compared to the test sample by simple visual inspection.