专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-11618777-B2 优先权日:2015-07-31 标 题 :Method of manufacturing membrane protein and utilization thereof 发明人:YOKOYAMA SHIGEYUKI; SHINODA TAKEHIRO; ITO KAORI; NOMURA NAOKO; KATSURA YOSHIKO; SOMEYA TOMOMI; SHIROUZU MIKAKO; HORI TETSUYA; NAKAMURA YOSHIHIRO; TANABE HIROAKI 权利人:YOKOYAMA SHIGEYUKI 摘要:In order to provide a membrane protein production method which does not require the step of solubilizing a membrane protein and which allows the membrane protein having an excellent quality to be obtained with a high yield, a method in accordance with an embodiment of the present invention includes: a step (a) of preparing a reaction solution for cell-free protein synthesis, the reaction solution containing (i) a template nucleic acid which encodes the membrane protein, (ii) a lipid, and (iii) a detergent which is contained at a concentration equal to or higher than a critical micelle concentration; and a step (b) of synthesizing the membrane protein while the concentration of the detergent in the reaction solution is maintained at a concentration equal to or higher than a critical micelle concentration.
专利号:US-2004220137-A1 优先权日:2001-07-07 标 题:Cosmetic and dermatological preparations containing osmolytes for the treatment of and active prevention of dry skin and of other negative alterations in the physiological homeostasis of healthy skin 发明人:SAUERMANN GERHARD 摘要:Use of n a) one or more compounds from the group of osmolytes or precursors and derivatives thereof, n b) optionally one or more compounds from the group of electrolytes and n c) optionally one or more compounds from the group formed by polyols and urea, n in preparations (cosmetics, dermatological preparations) to be applied topically for the treatment and active prevention of dry skin and for strengthening the barrier function of the skin, and for the treatment, care and prophylaxis of sensitive skin and/or for the treatment and prophylaxis of the symptoms of a negative change in the physiological homeostasis of healthy skin, in particular deficient, sensitive or hypoactive skin conditions or n deficient, sensitive or hypoactive conditions of skin appendages, inflamed skin conditions, and of atopic eczema, polymorphous photodermatosis, psoriasis, vitiligo, n sensitive, itching or irritated skin, n changes in normal lipid peroxidation, n a change in the ceramide, lipid and energy metabolism of healthy skin, n a change in the physiological transepidermal water loss, n a reduction in skin hydration and decrease in the moisture content of the skin, n change in the natural moisturizing factor content, n reduction in cell-cell communication, n deficiency symptoms of intracellular DNA synthesis, n DNA damage and reduction in endogenous DNA repair mechanisms, n activation of metalloproteinases and/or other proteases or inhibition of the corresponding endogenous DNA repair mechanisms, n deviations from the normal post-translational modifications of connective tissue constituents, n changes in the normal hyaluronic acid and glucosaminoglycan content of healthy skin, dandruff formation by the hair, flaking of the scalp and skin ageing.
专利号:US-11744248-B2 优先权日:2017-08-20 标 题:Disinfectant composition for control of clostridium difficile spore 发明人:SMYTH BRUCE; BRALKOWSKI MICHAEL 权利人:SMYTH BRUCE; BRALKOWSKI MICHAEL; ENVIRO SPECIALTY CHEMICALS INC 摘要:A composition and method for inducing and inflicting damage to cell membranes of microorganism by inducing changes in membrane permeability caused by polycation-induced membrane pores. The control of Clostridium difficile spores with an efficacy of 99.99% on hard surfaces is provided. The invention includes the application of an evaporation-induced self-assembly, surfactant-mediated synthesis or self-condensing polymer, which forms a film which is both flexible on the surface as a nano-polymeric coating and with refractive index yielding a transparent coating. The polymeric matrix includes various biocidal polymeric cationic quaternary salts, one being a silyl quaternary having a kinetic zeta potential to disrupt bacterial spores functions. The polymeric matrix consist of multiple small molecule biocides as well as polymerics biocide combinations not otherwise seen to develop an surface topography of peaks and troughs in the nano range to resist bacterial adhesion factors and subsequent biofilm formation. The surfactants induce self-assembly film topography.
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:EP-1848812-A2 优先权日:2004-10-01 标题:Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
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合成参考文献
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