专利号:US-7329515-B2 优先权日:2003-04-21 标题 :Solid support for the synthesis of 3′-amino oligonucleotides 发明人:LEUCK MICHAEL; WOLTER ANDREAS 权利人:SIGMA ALDRICH CO 摘要:The present invention discloses novel methods and solid supports for the synthesis of 3′-amino oligonucleotides. The novel supports are based on an unsubstituted or ring-substituted hydroxymethylbenzoyl linker element wherein the hydroxymethyl group is esterified to a solid phase bound carboxylic acid and the carbonyl group is linked to an amino alcohol as an amide. Oligonucleotides are conveniently synthesized on the novel supports with no modifications in the standard phosphoramidite synthesis scheme. The ester function of the support is cleaved under the alkaline deprotection conditions for oligonucleotides to provide a free hydroxymethyl group that aids in the release of the 3′-amino oligonucleotide products with a free amino group through neighbor group participation. The free amino group of the oligonucleotides is available for further conjugation reactions to haptens, reporter groups, surfaces or other small molecules or biomolecules. The methods provided are particularly mild, do not require any modifications in standard protocols for the synthesis and deprotection of oligonucleotides, provide the 3′-amino oligonucleotides free of side products and do not introduce chiral centers to the oligonucleotides.
专利号:US-8519184-B2 优先权日:2010-07-05 标 题:Synthesis of rare earth metal extractant 发明人:SAKAKI KAZUAKI; SUGAHARA HIROTO; OHASHI TETSUYA; KUME TETSUYA; IKKA MASAHIKO; NAGANAWA HIROCHIKA; SHIMOJO KOJIRO 权利人:SAKAKI KAZUAKI; SUGAHARA HIROTO; OHASHI TETSUYA; KUME TETSUYA; IKKA MASAHIKO; NAGANAWA HIROCHIKA; SHIMOJO KOJIRO; SHINETSU CHEMICAL CO; NISSIN CHEMICAL IND 摘要:A rare earth metal extractant in the form of a dialkyl diglycol amic acid is synthesized by reacting diglycolic anhydride with a dialkylamine in a synthesis medium. A molar ratio (B/A) of dialkylamine (B) to diglycolic anhydride (A) is at least 1.0. A non-polar or low-polar solvent in which the dialkyl diglycol amic acid is dissolvable is used as the synthesis medium.
专利号:EP-1749870-B1 优先权日:2005-08-04 标 题:Fluorescein-based compounds and their use for peptide synthesis 发明人:BALAKIREV MAXIM; BURCHAK OLGA 权利人:COMMISSARIAT ENERGIE ATOMIQUE 摘要:The present invention is related to new fluorescein derivatives, the method for producing such derivatives and their use for the synthesis of fluorogenic peptides and in particular protease substrates and peptide ligands.
专利号:US-6890732-B1 优先权日:1998-05-08 标题 :Nucleic acid molecules which code for enzymes derived from wheat and which are involved in the synthesis of starch 发明人:LOERZ HORST; LUETTICKE STEPHANIE; BLOCK MARTINA 权利人:BAYER CROPSCIENCE GMBH 摘要:Nucleic acid molecules are described which encode wheat enzymes involved in starch synthesis in plants. These enzymes are soluble wheat starch synthases. The invention furthermore relates to vectors and host cells which contain the above-described nucleic acid molecules. In particular to transformed plant cells and plants which can be regenerated from these and which have an increased or reduced activity of the soluble starch synthases according to the invention.
专利号:US-11945764-B2 优先权日:2021-06-09 标 题:Efficient synthesis of diglycolamide molecules 发明人:JANSONE-POPOVA SANTA; POPOVS ILJA 权利人:UT BATTELLE LLC 摘要:A method for producing a diglycolamide molecule having the formula: n nwherein R 1 and R 2 are independently selected from alkyl groups (R) and acyl groups (C(O)R) in which the alkyl groups (R) contain 1-30 carbon atoms and optionally contain an ether or thioether linkage between carbon atoms, and R 5 and R 6 are independently selected from hydrogen atom and alkyl groups containing 1-3 carbon atoms; and one or both pairs of R 1 and R 2 are optionally interconnected to form a ring; the method comprising: combining a diglycolic acid molecule (A) and a secondary amine (B) to form a salt intermediate (C), and heating the salt intermediate (C) to a temperature of 100° C. to 300° C. to form the diglycolamide of Formula (1) in a dehydration process, wherein the method is shown schematically as follows:
专利号:US-11920040-B2 优先权日:2018-05-29 标 题 :Carborhodamine compounds and methods of preparation thereof 发明人:LUKHTANOV EUGENY A 权利人:ELITECHGROUP MDX LLC 摘要:The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.
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合成参考文献
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