专利号:WO-2023227913-A1 优先权日:2022-05-27 标 题:Creating nucleic acids for in-vitro protein synthesis 发明人:CHEN MICHAEL CHUN HAO; MCINROY GORDON ROSS; OST TOBIAS WILLIAM BARR 权利人:NUCLERA LTD 摘要:Provided herein are methods, and compositions for the synthesis of genes and proteins. The methods are applicable to synthesis of nucleic acids and proteins on a microfluidic device.
专利号:WO-2024003538-A1 优先权日:2022-06-27 标题:Protein binding assays 发明人:CHEN MICHAEL CHUN HAO; CHEN SIHONG 权利人:NUCLERA LTD 摘要:Provided herein are methods, and compositions for the synthesis of proteins. The methods are applicable to synthesis of proteins on a microfluidic device and assays using the expressed proteins.
专利号:US-2024199533-A1 优先权日:2021-01-29 标题:Novel synthesis of salcaprozic acid by amide formation
专利号:WO-2024236295-A1 优先权日:2023-05-15 标题 :A method to homogenize the concentration of beads across multiple liquid volumes 发明人:GABER ZACHARY; KALSI SUMIT; LILLEY JOHNATHON; WAEBER ANDREAS MICHAEL 权利人:NUCLERA LTD 摘要:The invention provided herein relates to methods for normalising the concentration of beads in droplets on a microfluidic device, and methods for cell-free protein synthesis and characterisation of the expressed proteins.
专利号:EP-4284774-B1 优先权日:2021-01-29 标 题:Novel synthesis of salcaprozic acid by amide formation 发明人:KROGSGAARD-LARSEN NIELS; RAUNKJAER MICHAEL; RISINGER CHRISTIAN WALTER; TAANING ROLF HEJLE; KOMNATNYY VITALY 权利人:NOVO NORDISK AS
专利号:US-7544833-B2 优先权日:2006-09-07 标题 :Methods for producing N-(8-[2-hydroxybenzoyl]-amino) caprylic acid 发明人:BAY WILLIAM ELLIOTT; BERNADINO JOSEPH NORMAN; KLEIN GEORGE FREDERICK; REN YI; ZHANG PINGSHENG 权利人:HOFFMANN LA ROCHE; EMISPHERE TECH INC 摘要:Disclosed are improved methods for the synthesis of N-(8-[2-hydroxybenzoyl]-amino)caprylic acid. Certain compounds have been found useful for preventing the formation of a colored impurity when included in an ester hydrolysis reaction. Conducting ester hydrolysis in anaerobic conditions has also been found to minimize the formation of the color impurity. Also disclosed are improved methods for synthesizing the sodium salt of N-(8-[2-hydroxybenzoyl]-amino)caprylic acid.
1: Kang C, Bernaldez M, Stamatis SD, Rose JP, Sun R. Interaction between Permeation Enhancers and Lipid Bilayers. J Phys Chem B. 2024 Feb 22;128(7):1668-1679. doi: 10.1021/acs.jpcb.3c06448. Epub 2024 Jan 17. 24(8):221. doi: 10.1208/s12249-023-02676-8. 15(12):1585. doi: 10.3390/ph15121585. 4: Mortensen JS, Bohr SS, Harloff-Helleberg S, Hatzakis NS, Saaby L, Nielsen HM. Physical and barrier changes in gastrointestinal mucus induced by the permeation enhancer sodium 8-[(2-hydroxybenzoyl)amino]octanoate (SNAC). J Control Release. 2022 Dec;352:163-178. doi: 10.1016/j.jconrel.2022.09.034. Epub 2022 Oct 19. 626:122131. doi: 10.1016/j.ijpharm.2022.122131. Epub 2022 Aug 24. 23(6):171. doi: 10.1208/s12249-022-02328-3. 19(1):124-137. doi: 10.1021/acs.molpharmaceut.1c00689. Epub 2021 Dec 16.
合成参考文献
参考文献:10.1208/s12248-021-00670-1 摘要:Metry M, Polli JE. Evaluation of Excipient Risk in BCS Class I and III Biowaivers. The AAPS Journal. 2022 Jan 05;24(1):20. doi: 10.1208/s12248-021-00670-1.