📜3-氯丙基二甲基乙氧基硅烷置于氨体系中,化学反应生成(3-氨基丙基)二甲基乙氧基硅烷 参考文献:Notes. Preparation Of 3-Triethoxysilylpropylamine And 1,3-Bis(3-Aminopropyl)Tetramethyldisiloxane 标题:Notes. Preparation Of 3-Triethoxysilylpropylamine And 1,3-Bis(3-Aminopropyl)Tetramethyldisiloxane 摘要: Doi:10.1021/jo01083A612
专利号:US-2023323449-A1 优先权日:2021-12-17 标 题:Compositions and methods for detection of variants 发明人:GANTT RICHARD; ARNESON ADRIANA 权利人:TWIST BIOSCIENCE CORP 摘要:Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of ‘construction’ oligonucleotides and tagged complementary ‘selection’ oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.
专利号:US-9981239-B2 优先权日:2015-04-21 标题:Devices and methods for oligonucleic acid library synthesis 发明人:BANYAI WILLIAM; PECK BILL JAMES; FERNANDEZ ANDRES; CHEN SIYUAN; INDERMUHLE PIERRE; MARSH EUGENE P 权利人:TWIST BIOSCIENCE CORP 摘要:Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
专利号:WO-2024163733-A1 优先权日:2023-02-01 标题:Electrochemical synthesis with redox stable nucleotides 发明人:WU TIANDI; LACKEY JEREMY; LIN YANYOU; PITSCH STEFAN 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for constructing and storing polynucleotides encoding information with redox resistant bases. The compositions, devices, systems, and methods described herein provide for storage or synthesis of a library comprising a plurality of polynucleotides with one or more redox resistance bases. Further provided herein are methods to increase DNA synthesis yield and fidelity.
专利号:US-11512347-B2 优先权日:2015-09-22 标 题 :Flexible substrates for nucleic acid synthesis 发明人:PECK BILL JAMES 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Additionally, devices described herein for de novo synthesis of oligonucleic acids encoding information related to the original source information may have a flexible material for oligonucleic acids extension.
专利号:US-9808768-B2 优先权日:2012-09-24 标题:Method for synthesis of thin film composite membranes 发明人:VANKELECOM IVO; DOM ELLEN; KOECKELBERGHS GUY; HERMANS SANNE 权利人:UNIV LEUVEN KATH 摘要:The present invention relates to improved methods for the preparation of thin film composite membranes by interfacial polymerization, preferably (organic) solvent resistant thin film composite membranes. More in particular the method of the present invention allows for the preparation of thin film composite membranes wherein a thin film is deposited on a porous crosslinked support. Said method comprises the one step solidification, impregnation and crosslinking of the porous support through phase inversion by immersion of the cast membrane polymer film in an (aqueous) solvent comprising a polyfunctional monomers.
专利号:US-2023158469-A1 优先权日:2021-11-01 标题 :Devices and methods for synthesis 发明人:LACKEY JEREMY; DODD DAVID; PITSCH STEFAN 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for DNA oligomer synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.). The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.
参考文献:10.1007/978-1-62703-263-6_8 摘要:Ilisz I, Aranyi A, Pataj Z, Péter A. Enantioseparations by high-performance liquid chromatography using macrocyclic glycopeptide-based chiral stationary phases: an overview. Methods Mol Biol. 2013;970():137–63. doi: 10.1007/978-1-62703-263-6_8.