CAS: 74299-50-6; Sodium ((2R,3S,5R)-5-(6-Amino-9H-Purin-9-yl)-3-Hydroxytetrahydrofuran-2-yl)Methyl Dihydrogentriphosphate

专利信息

专利号:US-2013053252-A1
优先权日:2009-09-25
标题:Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
发明人：XIE XIAOLIANG SUNNEY; SIMS PETER A; GREENLEAF WILLIAM J; DUAN HAIFENG
权利人:XIE XIAOLIANG SUNNEY; SIMS PETER A; GREENLEAF WILLIAM J; DUAN HAIFENG; HARVARD COLLEGE
摘要:In general, the invention features methods and systems for sequencing of nucleic acids based on the measurement of the incorporation of fluorogenic nucleotides in microreactors. The invention provides numerous advantages over previous systems such as unambiguous determination of sequence, fast cycle time, long read lengths, low overall cost of reagents, low instrument cost, and high throughput. The invention also features methods and kits for nucleic acid amplification. The amplification and sequencing aspects of the invention may or may not be employed in conjunction with one another. The invention also features fluorogenic nucleotides that may be used in the sequencing methods of the invention.