CAS: 41335-32-4; 1-Naphthyl B-D-Galactopyranoside

上下游产品

α-naphthol β-D-galactose peracetate (2R,3S,4S,5R,6S)-2-(acetoxymethyl)-6-(naphthalen-1-yloxy)tetrahydro-2H-pyran-3,4,5-triyl triacetate

1-Naphthalenyl 2,3,4,6-Tetra-O-Acetyl-α/β-D-Galactopyranoside置于甲醇,Sodium Methylate体系中,化学反应生成 1-萘基-β-D-吡喃半乳糖苷
参考文献:基于结构的糖模拟fmlh配体在尿路感染过程中作为细菌粘附抑制剂的发现[微生物学]
标题:基于结构的糖模拟fmlh配体在尿路感染过程中作为细菌粘附抑制剂的发现[微生物学]
摘要:由于在全球范围内传播多药抗生素耐药性,细菌感染的治疗正成为一项严重的临床挑战,因此有必要寻找替代疗法来消除这些感染的致病机制.泌尿道致病性大肠杆菌(upec)使用多种分子伴侣-胶合剂通路菌毛,其中的黏附素具有不同的受体特异性,可以在各种宿主组织和生境中定殖.例如,Upec F9菌毛特异性结合在肾脏和发炎的膀胱上的半乳糖或n-乙酰半乳糖胺表位.使用x射线结构指导方法,虚拟筛选和多重elisa阵列,我们合理设计了芳基半乳糖苷和n抑制f9菌毛粘附素fmlh的-乙酰半乳糖氨基糖苷.铅化合物29β-Nac是具有k I的联苯基n-乙酰基-β-半乳糖氨基糖苷大约90 Nm,相对于大多数碳水化合物-凝集素相互作用的特征较弱的特性,其效能有了很大的提高.29β-Nac通过与a46环通过面对面π堆叠与残基y46,与羧酸酯基团进行盐桥相互作用的r142和与水通过氢键结合的k132与残基y46紧密结合,从而与fmlh紧密结合.
DOI:10.1073/pnas.1720140115

专利信息

专利号:US-5362625-A
优先权日:1991-05-15
标 题:Methods and compositions for enzyme complementation assays using the omega region of β-galactosidase
发明人：KREVOLIN MARK; KATES DAVID
权利人:MICROGENICS CORP
摘要:The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the beta -galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.

专利号:US-5643734-A
优先权日:1984-03-01
标题 :Methods for protein binding enzyme complementation assays
发明人：HENDERSON DANIEL ROBERT
权利人:MICROGENICS CORP
摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

专利号:US-5120653-A
优先权日:1985-04-08
标 题:Vector comprising DNA sequence coding for enzyme-donor polypeptide
发明人：HENDERSON DANIEL R
权利人:MICROGENICS CORP
摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

专利号:EP-0199801-B1
优先权日:1984-10-29
标 题:Methods for protein binding enzyme complementation assays
摘要:Improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristics of beta-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

专利号:US-4708929-A
优先权日:1984-10-29
标 题:Methods for protein binding enzyme complementation assays
发明人：HENDERSON DANIEL R
权利人:MICROGENICS CORP
摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.