CAS: 3368-04-5; 4-Methylumbelliferyl Phosphate

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CAS号1352006-36-0 dibenzyl 4-meth... | CAS号90-33-5 4-甲基伞形酮 | CAS号108-46-3 间苯二酚 | CAS号90-33-5 4-甲基伞形酮

📜Dibenzyl 4-Methyl-2-Oxo-2H-Chromen-7-Yl Phosphate置于palladium 10% On Activated Carbon,氢气体系中,用 甲醇 作为反应溶剂,化学反应 10.0H,反应生成 4-甲基伞形酮磷酸酯
参考文献:取代的2-氧代-2H-铬--7-基-二氢磷酸酯衍生物的合成和α-葡萄糖苷酶抑制,Dpph清除活性
标题:取代的2-氧代-2H-铬--7-基-二氢磷酸酯衍生物的合成和α-葡萄糖苷酶抑制,Dpph清除活性
摘要:通过pechmann缩合,磷酸化和脱苄基反应合成了一系列磷酸化香豆素衍生物(4A,4B,4C,4D,4E,4F,4G,4H,4I,4J),收率很高.因此,合成的化合物(4A,4B,4C,4D,4E,4F,4G,4H,4I,4J)评估了它们的α-葡萄糖苷酶和1,1-二苯基-2-吡咯并肼基清除活性; 几乎没有化合物显示出中等至非常好的活性.杂环化学杂志,00,00(2011).
DOI:10.1002/jhet.675

专利信息

专利号:US-10927135-B2
优先权日:2017-08-03
标 题:Metal-free direct arylation of dialkyl phosphonates for the synthesis of mixed alkyl aryl phosphonates
发明人：KANG JUN YONG; HUANG HAI
权利人:THE BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION ON BEHALF OF THE UNIV OF NEVADA LAS VE
摘要:Provided herein are phosphates, thiophosphates, phosphonates, and phosphinates, methods of making same, and methods of using these compounds and methods for the generation of pharmaceutically relevant phosphate, phosphonate, and phosphinate analogs. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

专利号:US-5164495-A
优先权日:1991-09-18
标题 :Method for preparing a dicarboxylic acid half-acid ester of FK506
发明人：LUNETTA STEVEN E
权利人:ABBOTT LAB
摘要:Disclosed is an improved method for the synthesis of FK506-hemisuccinate for utilization in the preparation of components for as assay for FK506.

专利号:US-9963735-B2
优先权日:2007-08-17
标题 :Selective 5′ ligation tagging of RNA
发明人：JENDRISAK JEROME; DAHL GARY A; VAIDYANATHAN RAMESH
权利人:EPICENTRE TECH CORPORATION
摘要:The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′ tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

专利号:US-7223541-B2
优先权日:2001-08-29
标 题:Terminal-phosphate-labeled nucleotides and methods of use
发明人：FULLER CARL; KUMAR SHIV; SOOD ANUP; NELSON JOHN
权利人:GE HEALTHCARE BIO SCIENCES
摘要:The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

专利号:US-6025477-A
优先权日:1986-03-31
标题 :Atherosclerotic plaque specific antigens, antibodies thereto, and uses thereof
发明人：CALENOFF EMANUEL
摘要:This invention provides purified antigens which are indicative of the presence of atherosclerotic plaque. Different concentrations of these antigens have been found to coincide with the progression of atherosclerosis. The subject invention also provides different hybridoma cell lines which produce monoclonal antibodies directed to antigens associated with atherosclerosis and a hybridoma cell line which produces monoclonal antibodies directed to antigen associated with normal artery and not with plaque. The atherosclerotic plaque antigen, and monoclonal antibodies made thereto, are used in various methods for detecting in a biological sample an antigen present in, and indicative of the presence of, atherosclerotic plaque. The monoclonal antibodies are also used in methods of imaging atherosclerotic plaque, and treating atherosclerosis. The methods of treating atherosclerosis include a method of digesting atherosclerotic plaque with enzymes, and a method of ablating atherosclerotic plaque using radiation. The subject invention also provides a method of treating atherosclerotic plaque by directly delivering a drug to the plaque. The subject invention further provides a method for treating atherosclerosis by blocking the synthesis of atherosclerotic plaque or by blocking binding of antibodies to the antigen.

专利号:US-9376707-B2
优先权日:2005-02-25
标题 :Kit for determination of thymidine kinase activity and use thereof
发明人：GRONOWITZ J SIMON
权利人:BIOVICA INTERNAT AB
摘要:An assay kit for determination of thymidine kinase (TK) activity in a biological sample, such as blood, serum, plasma, Cerebral Spinal Fluid (CSF), pleural fluid, ascites, tissues, cells and extracts thereof, is described. The assay kit can used in a method that comprises contacting, in a buffer, a Basic Reaction Mixture comprising: solid surface-attached primer and/or template, a modified deoxy nucleoside, such as BromodeoxyUridine, IododeoxyUridine, FluorodeoxyUridine or VinyldexoyThymidine as a kinase enzyme substrate, a phosphate donor, a nucleotide polymerizing enzyme, and a kinase enzyme source devoid of TK activity, such as a yeast extract, with the biological sample. After incubation the amount of modified deoxy nucleoside that has been incorporated into the solid surface-attached primer and/or template, is determined and the TK activity present in the biological sample is directly proportional to the amount of incorporated modified deoxy nucleoside. The assay kit are useful in the diagnosing, prognosis monitoring of disease progression and treatment effects of cell-proliferation disorders or diseases, such as cancer, and in the screening of compounds, e.g. new drug candidates, affecting enzymatic pathways, which may obstruct the formation of thymidine phosphates or interfere with nucleic acid synthesis.