专利号:US-2010324266-A1 优先权日:2000-09-11 标题:Photocleavable Protecting Groups 发明人:BARONE ANTHONY D; MCGALL GLENN H 权利人:AFFYMETRIX INC 摘要:Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 1 . Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
专利号:US-2011028350-A1 优先权日:1995-12-15 标题:Photocleavable protecting groups 发明人:MCGALL GLENN H; BARONE ANTHONY D 权利人:AFFYMETRIX INC 摘要:Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 5. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
专利号:US-11725222-B2 优先权日:2016-12-01 标题:Cleavable nucleotide analogs and uses thereof 发明人:GRISWOLD JR KETTNER JOHN FREDERICK; KOHMAN RICHARD E; CHURCH GEORGE M; RITTICHIER JONATHAN 权利人:HARVARD COLLEGE 摘要:Cleavable nucleotide analogs are provided. The nucleotide analog includes a nucleotide molecule attached to a cleavable moiety wherein the cleavable moiety comprises a protective group and/or a linker attached to a fluorophore. The cleavable moiety is linked to the oxygen atom of the 3′-OH of the pentose of the nucleotide molecule. The nucleotide analogs can be used in making polynucleotide molecules using template independent polymerases. The nucleotide analogs can act as reversible terminators during DNA sequencing by synthesis. The cleavage of the cleavable moiety restores a free 3′-OH functional group allowing growth of the polynucleotide molecule. The general structures as well as proposed synthetic schemes for the nucleotide analogs are also provided.
参考文献:10.1021/acs.jmedchem.9b00688 摘要:Li Z, Su K, Jiang Z, Yu Y, You Q, Zhang X. Photoactivatable Prolyl Hydroxylase 2 Inhibitors for Stabilizing the Hypoxia-Inducible Factor with Light. J Med Chem. 2019 Aug 22;62(16):7583–8. doi: 10.1021/acs.jmedchem.9b00688.