专利号:US-9113653-B2 优先权日:2011-08-19 标题:Methods of administering probiotic organisms that synthesize carotenoid compounds in situ to enhance human health and nutrition 发明人:MARANZ STEVEN J 权利人:MARANZ STEVEN J 摘要:A method is disclosed for increasing systemic carotenoid levels in humans through oral consumption of tablets, capsules, food or beverage products containing microbial organisms capable of synthesizing carotenoid compounds. Carotenoid synthesis may occur in situ by microbial organisms colonizing the gut, or in food or beverage products where carotenoids accumulate and are subsequently consumed dietarily. The microbial organisms may be either transgenic—thus expressing genes in the carotenoid synthesis pathway derived from other microbial organisms, or they may comprise species or strains selected for natural synthesis of carotenoids. The central objective of the invention is to provide an improvement in human health through a novel method for supplying and delivering carotenoid compounds.
专利号:US-2004097717-A1 优先权日:2000-03-24 标 题:Control of aerial branching 发明人:LEYSER OTTOLINE; BOOKER JONATHAN; SOREFAN KARIM 摘要:A plant nucleic acid sequence is provided which encodes a protein involved in the synthesis of abscisic acid. The plant nucleic acid sequence, and proteins encoded thereby, are useful in the regulation of aerial branching in plants.
专利号:US-11661594-B2 优先权日:2020-03-09 标 题:Mutant of lycopene epsilon cyclase (LCYE) gene crucial in wheat carotenoid synthesis pathway and use thereof 发明人:ZHAI SHENGNAN; LIU JIANJUN; LI HAOSHENG; SONG JIANMIN; LIU AIFENG; CAO XINYOU; CHENG DUNGONG; ZHAO ZHENDONG; LIU CHENG; GUO JUN; HAN RAN; ZI YAN; LI FAJI; WANG XIAOLU 权利人:CROP RES INST SHANDONG ACAD AGRICULTURAL SCIENCES 摘要:The present disclosure discloses a mutant of a lycopene epsilon cyclase (Lcye) gene crucial in a wheat carotenoid synthesis pathway and use thereof. The present disclosure provides the following proteins: (1) a protein obtained by substituting serine at position 253 of an Lcye-D1 protein with phenylalanine; (2) a derived protein that is obtained by subjecting the protein in (1) to substitution and/or deletion and/or addition of one or more amino acid residues and has the same ability as the protein in (1); (3) a protein that has a homology of more than 99%, more than 95%, more than 90%, more than 85%, or more than 80% with the amino acid sequence defined in any one of (1) and (2) and has the same function as the amino acid sequence; and (4) a fusion protein obtained by attaching a tag to N-terminus and/or C-terminus of the protein in any one of (1) to (3). The present disclosure not only verifies the function of an Lcye gene, but also provides a theoretical basis and a germplasm resource for improving the color character of flour and products thereof.
专利号:US-10590398-B2 优先权日:2015-06-04 标 题:Productivity and bioproduct formation in phototropin knock/out mutants in microalgae 发明人:NEGI SANGEETA; SAYRE RICHARD THOMAS; STARKENBURG SHAWN ROBERT 权利人:NMC INC; LOS ALAMOS NAT SECURITY LLC 摘要:Phototropin is a blue light receptor, which mediates a variety of blue-light elicited physiological processes in plants and algae. In higher plants these processes include phototropism, chloroplast movement and stomatal opening. In the green alga Chlamydomonas reinhardtii, phototropin plays a vital role in progression of the sexual life cycle and in the control of the eye spot size and light sensitivity Phototropin is also involved in blue-light mediated changes in the synthesis of chlorophylls, carotenoids, chlorophyll binding proteins. We compared the transcriptome of phototropin knock out (PHOT KO) mutant and wild-type parent to analyze differences in gene expression in high light grown cultures (500 μmol photons m−2 s−1). Our results indicate the up-regulation of genes involved in photosynthetic electron transport chain, carbon fixation pathway, starch, lipid, and cell cycle control genes. With respect to photosynthetic electron transport genes, genes encoding proteins of the cytochrome b6f and ATP synthase complex were up regulated potentially facilitating proton-coupled electron transfer. In addition genes involved in limiting steps in the Calvin cycle Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), Sidoheptulose 1,7 bisphosphatase (SBPase), Glyceraldehyde-3-phosphate dehydrogenase (3PGDH) and that mediate cell-cycle control (CDK) were also up regulated along with starch synthase and fatty acid biosynthesis genes involved in starch and lipid synthesis. In addition, transmission electron micrographs show increased accumulation of starch granules in PHOT mutant compared to wild type, which is consistent with the higher expression of starch synthase genes. Collectively, the altered patterns of gene expression in the PHOT mutants were associated with a two-fold increase in growth and biomass accumulation compared to wild type when grown in environmental photobioreactors (Phenometrics) that simulate a pond environment. In conclusion, our studies suggest that phototropin may be a master gene regulator that suppresses rapid cell growth and promotes gametogenesis and sexual recombination in wild type strains.
专利号:US-2023123612-A1 优先权日:2015-06-04 标题:Productivity and Bioproduct Formation in Phototropin Knock/Out Mutants in Microalgae 发明人:NEGI SANGEETA; SAYRE RICHARD THOMAS; STARKENBURG SHAWN ROBERT 权利人:NMC INC; LOS ALAMOS NAT SECURITY LLC 摘要:Phototropin is a blue light receptor, which mediates a variety of blue-light elicited physiological processes in plants and algae. In higher plants these processes include phototropism, chloroplast movement and stomatal opening. In the green alga Chlamydomonas reinhardtii , phototropin plays a vital role in progression of the sexual life cycle and in the control of the eye spot size and light sensitivity Phototropin is also involved in blue-light mediated changes in the synthesis of chlorophylls, carotenoids, chlorophyll binding proteins. We compared the transcriptome of phototropin knock out (PHOT KO) mutant and wild-type parent to analyze differences in gene expression in high light grown cultures (500 µmol photons m -2 s -1 ). Our results indicate the up-regulation of genes involved in photosynthetic electron transport chain, carbon fixation pathway, starch, lipid, and cell cycle control genes. With respect to photosynthetic electron transport genes, genes encoding proteins of the cytochrome b6f and ATP synthase complex were up regulated potentially facilitating proton-coupled electron transfer. In addition genes involved in limiting steps in the Calvin cycle Ribulose-1 ,5-bisphosphate carboxylase/oxygenase (RuBisCO), Sidoheptulose 1,7 bisphosphatase (SBPase), Glyceraldehyde-3-phosphate dehydrogenase (3PGDH) and that mediate cell-cycle control (CDK) were also up regulated along with starch synthase and fatty acid biosynthesis genes involved in starch and lipid synthesis. In addition, transmission electron micrographs show increased accumulation of starch granules in PHOT mutant compared to wild type, which is consistent with the higher expression of starch synthase genes. Collectively, the altered patterns of gene expression in the PHOT mutants were associated with a two-fold increase in growth and biomass accumulation compared to wild type when grown in environmental photobioreactors (Phenometrics) that simulate a pond environment. In conclusion, our studies suggest that phototropin may be a master gene regulator that suppresses rapid cell growth and promotes gametogenesis and sexual recombination in wild type strains.
专利号:US-2017211119-A1 优先权日:2014-07-25 标 题 :In vivo production of a recombinant carotenoid-protein complex 发明人:BOURCIER DE CARBON CÉLINE; KIRILOVSKY DIANA; BOULAY CLÉMENCE; DURAN BERTRAND-ELIE; PHULPIN ALEXANDRE; WILSON ADJÉLÉ 权利人:PHYCOSOURCE; Commissariat A L'Enerigie Atomique et Aux Energies Alteratives; CENTRE NAT DE LA RECH SCIENT (CNRS); COMMISSARIAT ENERGIE ATOMIQUE; CENTRE NAT RECH SCIENT 摘要:The present invention relates to a method for producing a carotenoid-protein complex in vivo comprising the steps of transforming of prokaryote cell with genes involved in carotenoid synthesis and with a gene encoding an apo-carotenoprotein; culturing said prokaryote cells such as to induce sequential genes expression, isolating and purifying the carotenoid-protein complex.
参考文献:10.1016/s0006-291x(80)80253-1 摘要:Camara B. Carotenoid biosynthesis in vitro conversion of violaxanthin to capsorubin by a chromoplast enriched fraction of capsicum fruits. Biochemical and Biophysical Research Communications. 1980 Mar;93(1):113–7. doi: 10.1016/s0006-291x(80)80253-1. 参考文献:10.1016/0005-2728(80)90202-9 摘要:Barrett J, Anderson JM. The P-700-chlorophyl a-protein complex and two major light-harvesting complexes of Acrocarpia paniculata and other brown seaweeds. Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1980 May;590(3):309–23. doi: 10.1016/0005-2728(80)90202-9. 参考文献:10.1073/pnas.96.15.8762 摘要:Havaux M, Niyogi KK. The violaxanthin cycle protects plants from photooxidative damage by more than one mechanism. Proc. Natl. Acad. Sci. U.S.A. 1999 Jul 20;96(15):8762–7. doi: 10.1073/pnas.96.15.8762. 参考文献:10.1073/pnas.96.15.8784 摘要:Lohr M, Wilhelm C. Algae displaying the diadinoxanthin cycle also possess the violaxanthin cycle. Proc. Natl. Acad. Sci. U.S.A. 1999 Jul 20;96(15):8784–9. doi: 10.1073/pnas.96.15.8784. 参考文献:10.1074/jbc.m602915200 摘要:Wehner A, Grasses T, Jahns P. De-epoxidation of Violaxanthin in the Minor Antenna Proteins of Photosystem II, LHCB4, LHCB5, and LHCB6. Journal of Biological Chemistry. 2006 Aug;281(31):21924–33. doi: 10.1074/jbc.m602915200.