专利号:US-2023212204-A1 优先权日:2020-01-16 标题:Reagents and their use for modular enantiodivergent synthesis of c-p bonds 发明人:XU DONGMIN; RIVAS-BASCÓN NAZARET; KNOUSE KYLE W; PADIAL NATALIA; ZHENG BIN; VANTOUROUT JULIEN; SCHMIDT MICHAEL; EASTGATE MARTIN D; BARAN PHI 权利人:BRISTOL MYERS SQUIBB CO; SCRIPPS RESEARCH INST 摘要:The disclosure describes chiral P(V)-based reagents and their uses for the modular, scalable, and stereospecific synthesis of chiral phosphines, phosphine oxides and particular oligonucleotides.
专利号:US-2014378538-A1 优先权日:2011-12-14 标 题:Methods of responding to a biothreat 发明人:BANCEL STEPHANE 权利人:MODERMA THERAPEUTICS INC 摘要:The present disclosure provides for devices, in particular mobile devices, which may be used in the synthesis of modified nucleic acid molecules, in particular modified mRNA molecules. The device for making the modified nucleosides, modified nucleotides and modified nucleic acids (e.g., mRNA) disclosed herein may be mobile devices comprising at least one sample block for insertion of one or more sample vessels, a device base with electronic control units for the sample block, a voltage supply, and one or more reagent(s) for the synthesis of at least one nucleic acid.
专利号:US-9790531-B2 优先权日:2012-08-14 标 题 :Enzymes and polymerases for the synthesis of RNA 发明人:WANG YUXUN; RAMAKRISHNAN DIVAKAR; DE FOUGEROLLES ANTONIN; WHORISKEY SUSAN 权利人:MODERNATX INC 摘要:The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmRNA molecules.
专利号:US-12031126-B2 优先权日:2020-05-08 标 题 :Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence 发明人:LIU DAVID R; ANZALONE ANDREW VITO; LEVY JONATHAN MA; GAO XIN; PODRACKY CHRISTOPHER J 权利人:BROAD INST INC; HARVARD COLLEGE 摘要:The present disclosure provides systems, compositions, and methods for simultaneously editing both strands of a double-stranded DNA sequence at a target site to be edited. In some aspects, the systems comprise a first and second prime editor complex, wherein each of the first and second prime editor complexes comprises (1) a prime editor comprising (i) a nucleic acid programmable DNA binding protein (napDNAbp), and (ii) a polypeptide having an RNA-dependent DNA polymerase activity; and (2) a pegRNA comprising a spacer sequence, gRNA core, a DNA synthesis template, and a primer binding site, wherein the DNA synthesis template encodes a desired DNA sequence or a complement thereof, wherein the desired DNA sequence and the complement thereof form a duplex comprising an edited portion which integrates into the target site to be edited. In some aspects, the systems comprise a first, second, third, and fourth prime editor complex, each comprising a prime editor and a PEgRNA. Also provided herein are methods for simultaneously editing both strands of a double-stranded DNA sequence at a target site to be edited. Further provided herein are pharmaceutical compositions, polynucleotides, vectors, cells, and kits.
专利号:US-11920174-B2 优先权日:2016-03-03 标 题 :RNA analysis by total hydrolysis and quantification of released nucleosides 发明人:EBER FABIAN JOHANNES; WOCHNER ANIELA; ROOS TILMANN 权利人:CureVac SE 摘要:The present invention relates to analysis of an RNA molecule. It further relates to the use of this method for the quality control of an RNA molecule produced by in vitro transcription or for the quality control of an RNA molecule produced by chemical synthesis.
专利号:CN-115244061-A 优先权日:2020-01-16 标题:Reagents and their use for modular enantio-divergent synthesis of C-P bonds
[参考文献]: Ohkawa Y, Et Al. Application Of 4-Hydroxyantipyrine And Acetaminophen O-Sulfate As Biodistribution Promoter. Biol Pharm Bull. 2001 Dec;24(12):1404-10. [参考文献]: Ohkawa Y, Et Al. Effects Of 4-Hydroxyantipyrine And Its 4-O-Sulfate On Antipyrine As Biodistribution Promoter. Biol Pharm Bull. 2001 May;24(5):529-34. [参考文献]: F Jorquera, Et Al. The Effect Of Age And Sex On Metabolism And Urinary Excretion Of Antipyrine. J Gerontol A Biol Sci Med Sci. 1998 Jan;53(1):M14-9. [参考文献]: G M Robertz-Vaupel, Et Al. Disposition Of Antipyrine In Patients With Extensive Metastatic Liver Disease. Eur J Clin Pharmacol. 1992;42(5):465-9. [参考文献]: H A Ali, Et Al. Elimination Studies Of Antipyrine And Its Metabolites In Healthy Saudi Arabians. Hum Exp Toxicol. 1994 Oct;13(10):658-62.
合成参考文献
参考文献:10.1139/y80-004 摘要:Inaba T, Fischer NE. Antipyrine metabolism in man: simultaneous determination of norantipyrine and 4-hydroxyantipyrine in urine by gas chromatography. Can J Physiol Pharmacol. 1980 Jan;58(1):17–21. doi: 10.1139/y80-004. 参考文献:10.1007/bf03190016 摘要:Danbury TC, Eccles M, Ford J, Roberts CJ. Effect of tacrine hydrochloride on hepatic drug metabolism. European Journal of Drug Metabolism and Pharmacokinetics. 1999 Mar;24(1):91–6. doi: 10.1007/bf03190016. 参考文献:10.1007/bf03189981 摘要:Mönig H, Hoffmann K, Ohnhaus EE, Schulte HM. Ranitidine treatment and cortisol metabolism in man. European Journal of Drug Metabolism and Pharmacokinetics. 1992 Jan;17(1):9–12. doi: 10.1007/bf03189981. 参考文献:10.1016/0378-4347(92)80180-x 摘要:Moreau J, Palette C, Cordonnier P, Naline E, Advenier C, Pays M. Separation and identification of the 4-hydroxyantipyrine sulphoconjugate. Journal of Chromatography B: Biomedical Sciences and Applications. 1992 Apr;576(1):103–9. doi: 10.1016/0378-4347(92)80180-x. 参考文献:10.1007/pl00013798 摘要:Mauriz JL, Tabernero B, García-López J, Jorquera F, Villa JG, González-Gallego J. Physical exercise and improvement of liver oxidative metabolism in the elderly. European Journal of Applied Physiology. 2000 Jan;81(1-2):62–6. doi: 10.1007/pl00013798.