CAS: 16481-54-2; Podophyllotoxin, 3-.β.-D-Glucopyranoside

上下游产品

CAS号186581-53-3 重氮甲烷 | CAS号133-89-1 尿苷二磷酸葡萄糖 | CAS号518-28-5 鬼臼毒素 | CAS号518-28-5 鬼臼毒素

📜尿苷(5')二氢二磷酰(1)-Alpha-D-葡萄糖,鬼臼毒素置于1,4-二巯基-2,3-丁二醇体系中,用 Various Solvents 用作溶剂,化学反应生成鬼臼毒素-4-O-葡萄糖苷
参考文献:Udp-Glucose:(6-Methoxy)Podophyllotoxin 7-O-Glucosyltransferase From Suspension Cultures Of Linum Nodiflorum
标题:Udp-Glucose:(6-Methoxy)Podophyllotoxin 7-O-Glucosyltransferase From Suspension Cultures Of Linum Nodiflorum
摘要:Cell Cultures Of Linum Species Store 6-Methoxypodophyllotoxin (Mptox),Podophyllotoxin (Ptox) And Related Lignans As O-Glucosides. Udp-Glucose:(M)Ptox 7-O-Glucosyltransferase Has Been Detected And Characterised In Protein Preparations Of Suspension-Cultured Cells Of Linum Nodiflorum L. (Linaceac). The Maximal Lignan Glucoside Contents In The Cells Are Preceded By A Rapid Increase Of The Specific Glucosyltransferase,Activity On Day Six Of The Culture Period. Mptox Glucoside Is The Major Lignan With Up To 1.18 Mg G(-1) Of The Cell Dry Wt Which Is More Than 30-Fold Of The Ptox Glucoside Content. Of The Three Aryltetralin Lignans Tested As Substrates,Ptox And Mptox Display Comparable Apparent K-M Values Of 4.7 And 5.4 Mu M,Respectively. 5'-Demethoxy-6-Methoxypodophyllotoxin Is Converted With The Highest Velocity Of 25.2 Pkat Mg(-1) While Also Possessing A Higher K-M Of 14.7 Mu M. Two-Substrate Test Series Indicate That All Three Compounds Compete For The Active Site Of A Single Protein. The Structurally Similar Lignan O-Peltatin Acts As Competitive Inhibitor As Well. However,The 6-O-Glucosidation Is Most Likely Catalysed By A Separate Enzyme. The (M)Ptox 7-O-Glucosyltransferase Works Best At A Ph Around 9 And A Temperature Around 35 Degrees C. A 15-30% Increase Of The Reaction Rate Is Effected By The Addition Of 0.9 Mm Mn2+. (C) 2007 Elsevier Ltd. All Rights Reserved.
Doi:10.1016/j.Phytochem.2007.07.030