专利号:US-3781324-A 优先权日:1969-11-03 标 题:Certain 6-trifluoromethylcytosines and thiocytosines,their synthesis,and their use in the synthesis of uracils and thiouracils 发明人:LUTZ A 权利人:AMERICAN CYANAMID CO 摘要:3-AMINO-2-SUBSTITUTED - 4,4,4 - TRIFLUORO-2-BUTENENITRILES ARE REACTED WITH ISOCYANATES OR ISOTHIOCYANATES IN THE PRESENCE OF AN INERT SOLVENT AND FROM 0.75 TO ABOUT 1.0 MOLE EQUIVALENTS OF STRONG BASE PER MOLE OF BUTENENITRILE TO PRODUCE THE NOVEL UREIDO BUTENENITRILES OF THE FORMULA: CF3-C(-NH-C(=X)-NH-R)=C(-HALO)-CN WHEREIN R IS ALKYL, ALKENYL, PHENYL, BENZYL AND SUBSTITUTED DERIVATIVES THEREOF AND X IS SULFUR OR OXYGEN. THE UREIDO BUTENENITRILE ARE TREATED WITH ADDITIONAL BASE TO PRODUCE NOVEL CYCLIC CYTOSINES OF THE FORMULA: 6-CF3,5-HALO,4-(HN=),3-R,2-(X=)-1,2,3,4-TETRAHYDRO- PYRIMIDINE WHEREIN R AND X ARE AS DEFINED ABOVE. THE CYTOSINES CAN BE PRODUCED DIRECTLY BY TREATING 3-AMINO-2-SUBSTITUTED4,4,4-TRIFLUORO-2-BUTENENITRILE WITH AT LEAST 1.0 MOLE EQUIVALENT OF BASE. THE NOVEL COMPOUNDS ARE USEFUL INTERMEDIATES IN THE PREPARATION OF HERBICIDAL URACILS AND THIOURACILS.
专利号:US-3635977-A 优先权日:1969-11-03 标 题 :Certain 6-trifluoromethylcytosines and thiocytosines their synthesis and their use in the synthesis of uracisls and thiouracil 发明人:LUTZ ALBERT WILLIAM 权利人:AMERICAN CYANAMID CO 摘要:3 - AMINO - 2 - SUBSTITUTED - 4,4,4 - TRIFLUORO - 2 - BUTENENITRILES ARE REACTED WITH ISCYANATES OR ISOTHIOCYANATES IN THE PRESENCE OF AN INERT SOLVENT AND FROM 0.75 TO ABOUT 1.0 MOLE EQUIVALENTS OF STRONG BASE PER MOLE OF BUTENENITRILE TO PRODUCE THE NOVEL UREIDO BUTENEITRILES OF THE FORMULA: NC-C(-HALO)=C (-CF3)-NH-C(=X)-NH-R PYRIMIDINE WHEREIN R IS ALKYL, ALKENYL, PHENYL, BENZYL AND SUBSITUTED DERIVATIVES THEREOF AND X IS SULFUR OR OXYGEN. THE UREIDO BUTENENITRILES ARE TREATED WITH ADDITIONAL BASE TO PRODUCE NOVEL CYCLIC CYTOSINES OF THE FORMULA; 2(=X),3-R,4-(=NH),5-(HALO-),6-(F3C-)-1,2,3,4-TETRAHYDRO WHEREIN R AND X ARE AS DEFINED ABOVE. THE CYTOSINES CAN BE PRODUCED DIRECTLY BY TREATING 3-AMINO-2-SUBSITUTED4,4,4-TRIFLUORO-2-BUTENENTRILE WITH AT LEAST 1.0 MOLE EQUIVLAENTS OF BASE. THE NOVEL COMPOUNDS ARE USEFUL INTERMEDIATES IN THE PREPARATION OF HERBICIDAL URACILS AND THIOURACILS.
专利号:US-5128419-A 优先权日:1990-08-20 标 题:Synthesis of tagged polymers by post-polymerization (trans) amidation reaction 发明人:FONG DODD W; HOOTS JOHN E 权利人:NALCO CHEMICAL CO 摘要:Polymers tagged with pendant fluorescent groups are prepared by the (trans)amidation derivatization of pre-existing polymers having carbonyl-type pendant groups. Polymers having pendant amide groups wherein the amide nitrogen is substituted with fluorescent moieties, prepared by (trans)amidation drrivatization, are provided. Polymers having pendant (sulfonated) napthalene moieties substituted to amide nitrogen are provided.
专利号:US-5260386-A 优先权日:1990-08-20 标 题 :Synthesis of tagged polymers by post-polymerization (trans) amidation reaction 发明人:FONG DODD W; HOOTS JOHN E 权利人:NALCO CHEMICAL CO 摘要:A polymer having pendant fluorescent groups is prepared by (trans)amidation of a preformed polymer by reaction with an amine-containing organic fluorescent composition of the Formula III Formula III wherein one of R5 and R6 may be hydrogen, and wherein within at least one of R5 and R6, or within R5 and R6 taken together, is an organic fluorescent group wherein the organic fluorescent group includes a polynuclear aromatic ring system. A degree of (trans)amidation derivatization of the polymer is accomplished by heating the admixture of polymer and fluorescent agent for a sufficient period of time. A second amine-containing post-polymerization derivatization agent other than the organic fluorescent composition of Formula III is added to the admixture or to at least a portion of the reaction product of the (trans)amidation derivatization, and is heated therewith to accomplish a degree of sequential or simultaneous post-polymerization derivatization with the second amine-containing post-polymerization derivatization agent.
专利号:US-5308460-A 优先权日:1992-10-30 标 题 :Rapid synthesis and analysis of carbohydrates 发明人:MAZID M ABDUL; KLOCK JOHN C 权利人:GLYKO INC 摘要:The subject invention provides novel methods and apparatus for synthesizing carbohydrates of a desired structure. The subject synthesis methods include using carbohydrage-modifying enzymes to perform individual synthesis reaction steps and electrophoresis to purify the reaction products for possible further use as substrates in additional synthesis reactions. The subject invention also provides novel methods and apparatus for detecting carbohydrates of interest. The methods and apparatus for synthesizing or detecting carbohydrates involve the use of carbohydrate-modifying enzymes immobilized in a reaction matrix gel and fluorophore labelled substrates that may be removed from the reaction matrix gel by electrophoresis and subsequently analyzed or transferred to another reaction gel matrix. n The subject invention also provides methods for detecting and quantitating specific carbohydrate-modifying enzymes that might be present in a sample for analysis.
专利号:US-2006252672-A1 优先权日:2005-04-05 标题 :Protein N-glycosylation of eukaryotic cells using dolichol-linked oligosaccharide synthesis pathway, other N-gylosylation-increasing methods, and engineered hosts expressing products with increased N-glycosylation 发明人:BETENBAUGH MICHAEL J; VISWANATHAN KARTHIK; KRAG SHARON S; JONES JULLIAN G 权利人:BETENBAUGH MICHAEL J; VISWANATHAN KARTHIK; KRAG SHARON S; JONES JULLIAN G 摘要:The level of glycosylation on products produced by a host (such as CHO cells, HEK cells and other mammalian cells, and non-mammalian cells) or patient can be increased by engineering, such as by supplying the host or patient with a gene sequence. For example, the host or patient can be made to produce desirably glycosylated products by increasing one or both of expression of N-glycan substrate containing lipid-liked oligosaccharide and expression of oligosaccharide (OST) transferase.
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