专利号:US-2004054162-A1 优先权日:2001-10-30 标 题:Molecular detection systems utilizing reiterative oligonucleotide synthesis 发明人:HANNA MICHELLE M 摘要:The present invention provides methods for detecting the presence of a target molecule by the use of nucleotide analogs containing moieties that enable detection. Such analogs may be incorporated into nucleic acids. In one embodiment, nucleotide analogs are used in a process generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators or chain terminators to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase.
专利号:US-2023314322-A1 优先权日:2022-03-29 标题:Systems and methods of sequencing polynucleotides 发明人:CALLINGHAM MICHAEL JAMES; BUREK MICHAEL JOHN; WINNARD CHRISTOPHER; MILLER OLIVER JON 权利人:ILLUMINA INC; ILLUMINA CAMBRIDGE LTD 摘要:The application relates to DNA sequencing systems and methods. Systems and methods for determining the nucleotide sequence of a polynucleotide include attaching three different fluorescent dyes to three different nucleotides during incorporation. In particular, long Stokes shifted dyes may be used to determine the sequence of polynucleotides in a sequencing by synthesis system.
专利号:WO-2018157153-A1 优先权日:2017-02-27 标 题:Large scale synthesis of messenger rna
专利号:WO-2013038134-A1 优先权日:2011-09-16 标题 :Synthesis and anticancer activity of ruthenium (11) cis-cis-1,3,5- triaminocyclohexane complexes 发明人:GAMBLE AIMEE JOANNA; LYNAM JASON MARTIN; WALTON PAUL HOWARD 权利人:UNIV YORK; GAMBLE AIMEE JOANNA; LYNAM JASON MARTIN; WALTON PAUL HOWARD 摘要:There is described a complex of formula I: in which, R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are each, hydrogen, alkyl CI to 20 alkenyl CI to 20, aryl, alkyl(ci t0 20)aryl, alkenyl(Ci t0 20>aryl, heteroaryl, etc.; R 7 , R 8 and R 9 are each, hydrogen or alkyl CI to 20; L 1 , L 2 and L 3 , are each a ligand; in free or in salt form.
专利号:WO-2018219107-A1 优先权日:2017-05-27 标题:Engineered polypeptides and their applications in synthesis of beta-hydroxy-alpha-amino acids
专利号:RU-2042668-C1 优先权日:1989-03-20 标 题:Method of synthesis of 9-substituted guanine derivatives and 1-substituted 5-(thiocarbamoyl) -amino-1h- imidazole-4- carboxamides
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合成参考文献
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