专利号:US-2023313255-A1 优先权日:2020-07-15 标题:Massively Parallel Enzymatic Synthesis of Polynucleotides 发明人:HORGAN ADRIAN; LACHAIZE HENRI; VERARDO DOMIANO; GODRON XAVIER 权利人:DNA SCRIPT 摘要:The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.
专利号:US-2023323449-A1 优先权日:2021-12-17 标 题:Compositions and methods for detection of variants 发明人:GANTT RICHARD; ARNESON ADRIANA 权利人:TWIST BIOSCIENCE CORP 摘要:Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of ‘construction’ oligonucleotides and tagged complementary ‘selection’ oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.
专利号:WO-2024153643-A1 优先权日:2023-01-16 标 题 :Inkjet-assisted enzymatic nucleic acid synthesis 发明人:MOGHADDAM NICOLAS; ADELIZZI BEATRICE; LOMAN TESSA; VERARDO DAMIANO; RODRIGUEZ-PINZON DANIEL; HORGAN ADRIAN 权利人:DNA SCRIPT 摘要:Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides.
专利号:WO-2024184538-A1 优先权日:2023-03-09 标 题:A microfluidic chip and method for synthesis of nanoparticles 发明人:ZHANG NAN; LIU DONGSHENG; YU MINGZHI; SHAH PRANAY; ALLEN MATTEW 权利人:UNIV DUBLIN 摘要:A microfluidic chip (1,100) microfluidic chip (1,100) comprising at least two inlets (2, 4, 4a-4j) that meet at a junction (5, 5a, 5b) that is in fluid communication with a proximal end (3) of a channel (8, 8a, 8b, 8c) thereof and an outlet (6) that is in fluid communication with a distal end (7) of the channel (8), wherein the channel (8, 8a, 8b, 8c) comprises either a plurality of spaced-apart baffle structures (10) continuous with an inner wall (12) of the channel (8, 8a, 8b, 8c) or at least one aerofoil-shaped baffle structure (14), or a combination thereof, and forms a microchannel (9) in the channel (8, 8a, 8b, 8c) adapted to accommodate a fluid.
专利号:WO-2024141628-A1 优先权日:2022-12-31 标 题:Variable viscosity inks for inkjet delivery of enzyme reagents 发明人:HORGAN ADRIAN; VERARDO DAMIANO; ADELIZZI BEATRICE 权利人:DNA SCRIPT 摘要:The invention is directed to formulations of terminal deoxynucleotidyl transferases (TdTs) inks employing variable viscosity modifiers and their use in inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate.
专利号:US-2013087020-A1 优先权日:2011-10-07 标题:Continuous flow synthesis of nanomaterials using ionic liquids in microfluidic reactors 发明人:BRUTCHEY RICHARD; MALMSTADT NOAH; LAZARUS LAURA; YANG ASTRO S-J; RICHE CARSON; CHU STEVEN; MARIN BRANDON 权利人:UNIV SOUTHERN CALIFORNIA; UNIV SOUTHERN CALIFORNIA 摘要:A method for manufacturing metal nanoparticles includes the use of a microfluidic device. The microfluidic device has a first channel having a first inlet; a second channel having a second inlet; a third channel having a third inlet; and a main channel having a main inlet and an outlet. The first channel, second channel, and third channel all lead into the main channel. The method involves injecting a solution of a metal/ligand into the first inlet, injecting a solution of a reducing agent into the second inlet, injecting a solvent comprised of an ionic liquid into the third inlet, and injecting an inert carrier into the main inlet. The solution of the metal/ligand, the solution of the reducing agent, the solvent and the inert carrier are combined together in the main channel, and the metal/ligand and the reducing agent are reacted for a time sufficient to form a metal nanoparticle.
[参考文献]: Andrea Pavesi, Et Al. Controlled Electromechanical Cell Stimulation On-A-Chip. Sci Rep. 2015 Jul 2:5:11800. [参考文献]: Francisco Palazon, Et Al. Orthogonal Chemical Functionalization Of Patterned Gold On Silica Surfaces. Beilstein J Nanotechnol. 2015 Dec 1:6:2272-7. [参考文献]: Huiyan Li, Et Al. A Versatile Snap Chip For High-Density Sub-Nanoliter Chip-To-Chip Reagent Transfer. Sci Rep. 2015 Jul 7:5:11688. [参考文献]: Lewis E Scott, Et Al. Fibronectin Fibrillogenesis Facilitates Mechano-Dependent Cell Spreading, Force Generation, And Nuclear Size In Human Embryonic Fibroblasts. Integr Biol (Camb). 2015 Nov;7(11):1454-65. [参考文献]: Nisarga Naik, Et Al. Rapid Homogeneous Endothelialization Of High Aspect Ratio Microvascular Networks. Biomed Microdevices. 2015 Aug;17(4):83.
合成参考文献
摘要:S25 | OECDPFAS | List of PFAS from the OECD | DOI:10.5281/zenodo.2648775 摘要:S125 | FLUOROIONS | Fluorinated Ionic Liquids Suspect List | DOI:10.5281/zenodo.15348301. 摘要:S124 | IONICLIQUIDS | Ionic Liquids Suspect List | DOI:10.5281/zenodo.15348268. 参考文献:10.1038/ncomms14346 摘要:Kim SH, Im SK, Oh SJ, Jeong S, Yoon ES, Lee CJ, Choi N, Hur EM. Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network. Nat Commun. 2017 Feb 01;8():14346. 参考文献:10.1038/s41592-019-0455-2 摘要:Manfrin A, Tabata Y, Paquet ER, Vuaridel AR, Rivest FR, Naef F, Lutolf MP. Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells. Nat Methods. 2019 Jul;16(7):640–8. doi: 10.1038/s41592-019-0455-2.