CAS: 64013-70-3; (2R,3S,4S,5R,6R)-2-(Aminomethyl)-6-(((1R,2R,3S,4R,6S)-4,6-Diamino-3-(((2S,3R,4S,5S,6R)-4-Amino-3,5-Dihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)-2-Hydroxycyclohexyl)Oxy)Tetrahydro-2H-Pyran-3,4,5-Triol Bis(Sulfate)

上下游产品

kananmycin B di-tert-butyl ((1S,3R,4S,5R,6R)-4-(((2S,3R,4S,5S,6R)-4-((tert-butoxycarbonyl)amino)-3,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-6-(((2R,3R,4S,5S,6R)-6-(((tert-butoxycarbonyl)amino)methyl)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)oxy)-5-hydroxycyclohexane-1,3-diyl)dicarbamate 6'-acetylkanamycin 6''-(2,4,6-triisopropylbenzenesulfonyl)-1,3,6',3''-tetra-N-(tert-butoxycarbonyl)kanamycin A

专利信息

专利号:WO-2024058717-A1
优先权日:2022-09-14
标题:Engineering of rhizomucor miehei lipase towards amide bond formation for the synthesis of medium to long chain n-acyl glycines in aqueous media
发明人：LI ZHI; KUA KAI BIN; NGUYEN KIEN TRUC GIANG
权利人:WILMAR INTERNATIONAL LTD; NAT UNIV SINGAPORE
摘要:Described herein are lipase variants of a Rhizomucor Miehei Lipase. The lipase variants may be used to prepare medium to long chain N-acyl glcyines. The variants may have a parent sequence of SEQ ID NO: 3; and a D156 substitution and/or a L258 substitution, wherein the D156 substitution is selected from the group consisting of serine (S), glycine (G), glutamic acid (E), and threonine (T), wherein if the D156 substitution is glycine, the lipase variant comprises at least one other substitution, and the L258 substitution is selected from the group consisting of lysine (K), arginine (R), glutamine (Q), asparagine (N), histidine (H), and serine (S). The variants may have a parent sequence of SEQ ID NO: 3; and at least one substitution of the following amino acids: D156, L258. L267, V254, L255, N264, T265, S83, S84, W88, R30, R86, S56, L58, and 1I59.