CAS: 9073-78-3; Thermolysin
该化合物是一种来自Bacillus热蛋白质的热能金属蛋白,在高温(通常为60-80°C)和对碱性pH(6.0-9.0)中中性(6.0-9.0)表现出最佳活动. 它的锌依赖催化机制能够有效地分离peptide 键,特别是涉及疏水残留的. 关键优势包括:大量非极地氨基酸(例如,利西内,苯丙烯)和有机溶剂稳定性高特质,因此对peptide合成和蛋白排序很有价值. 热解素的热稳定性降低了工业应用中的污染风险. 它被广泛用于生物技术过程,包括作为片的生产和结构生物学中有限的分解研究. 酶的坚固性和特性强调了其在研究和工业环境中的实用性.
- 英文名称Thermolysin
- 中文名称嗜热菌蛋白酶
- 其它别名Thermolysin; Thermophilic-Bacterial Protease; Protease Type X; Ec 3.4.24.2; Ec 3.4.24.27; Ec 3.4.24.37; Thermolysin From Geobacillus Stearothermophilus; Thermolysin Bacillus Thermoproteolyticus Rokko
- CAS编号9073-78-3
- MFCD编号:MFCD00165549
- EINECS号:232-973-4
- 分子式:CaH2Zn分子量:0.0
- 产品CID: 1940968
- 产品分类生物化工 → 酶及辅酶类
- 相似结构搜索
- InChIKey: ——
- 1S/Ca.Zn.2H
- 计算化学: 氢键受体数*氢键供体数*可旋转键数*
物理性质
- 敏感性Application A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C . Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala ; and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes. Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ci°Calteu reagent).
- 外观形态棕褐色或类白色粉末
- 储存条件-20°C
- 产品应用该化合物用于一项研究,调查氯化钠对热稳定性和催化活动的影响.
MSDS等安全信息
- GHS象形图
- GHS符号GHS07 & GHS08;
注释: Exclamation mark & Health hazard - 危险类别特定目标器官毒性 - 单次接触 类别3
皮肤腐蚀/刺激 类别2
严重眼刺激 类别2
呼吸道致敏 类别1
皮肤致敏 类别1 - 警示词Danger(危险)
- 危险描述H335 |可能引起呼吸道刺激.
H315 |造成皮肤刺激.
H319 |造成严重眼刺激.
H334 |吸入可能引起过敏或哮喘症状或呼吸困难.
H317 |可能引起皮肤过敏反应. - 防范说明P261-P284-P304+P340+P312-P501
- UN编号NONH for all modes of transport
- 危险品标志Xn
- 安全声明22-26-36/37
- 危险类别码36/37/38-42/43
- WGK Germany3
