CAS: 3546-41-6; 2-(2-(2,5-Dimethyl-1-Phenyl-1H-Pyrrol-3-yl)Vinyl)-6-(Dimethylamino)-1-Methylquinolin-1-Ium Bis-4,4'-Methylenebis(3-Hydroxy-2-Naphthoate)

上下游产品

(E)-2-[2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)vinyl]-1-methyl-6-(dimethylamino)quinolinium methyl sulphate salt embonic acid disodium salt 4-amino-N,N-dimethylaniline (E)-2-(2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)vinyl)-6-(dimethylamino)-1-methylquinolin-1-ium iodide

专利信息

专利号:US-7344863-B2
优先权日:1998-03-13
标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules
发明人：LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT
权利人:INVITROGEN CORP
摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

专利号:WO-9824022-A1
优先权日:1996-11-22
标题:Method for incremental synthesis of a discrete technical system
发明人：STEFFEN BERNHARD
权利人:METAFRAME TECHNOLOGIES SOFTWAR; STEFFEN BERNHARD
摘要:Disclosed is a method for knowledge-based incremental synthesis of a discrete technical system dependent upon a predetermined first configuration, wherein the first configuration represents an implemented consistent conflict-free causal structure. The first configuration comprises a predetermined knowledge base and a description of requirements controlling the synthesis, wherein a second configuration which fulfills the description of requirements is generated in the following steps: synthesis of a completed first intermediate causal structure that is not necessarily consistent or conflict-free, arising from the first configuration and the description of requirements by means of a fusion specification, and transformation of the first intermediate causal structure into a not necessarily conflict-free completed consistent second intermediate causal structure, and transformation of the completed second intermediate causal structure into a conflict-free consistent causal structure corresponding to the second configuration of the technical system by conflict resolution using conflict resolution conditions.

专利号:US-7122304-B2
优先权日:1997-05-12
标 题:Methods for the storage and synthesis of nucleic acids using a solid support
发明人：GOLDSBOROUGH MINDY D; FOX DONNA K
权利人:WHATMAN INC
摘要:The invention relates to storage of nucleic acid (particularly mRNA) on a solid support and to using such nucleic acid in nucleic acid synthesis or amplification reactions. In a preferred aspect, the invention provides synthesis of cDNA and cDNA libraries.

专利号:US-2007178489-A1
优先权日:1999-05-12
标 题:Compositions and Methods for Enhanced Sensitivity and Specificity of Nucleic Acid Synthesis
发明人：ASTATKE MEKBIB; CHATTERJEE DEB K; SHADILYA HARINI
权利人:INVITROGEN CORP
摘要:The present invention relates to polypeptides, compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses polypeptides having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis and more particularly to polymerases having reduced polymerase and optionally reduced exonuclease activities (3′ to 5′ and/or 5′ to 3′ exonuclease activity), and to nucleases having reduced nuclease activity. The polypeptides of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot startâ€? synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the polypeptides or compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to polypeptides and compositions useful for inhibiting or preventing degradation of various nucleic acid molecules.

专利号:US-8043816-B2
优先权日:1999-07-02
标题 :Compositions and methods for temperature-dependent nucleic acid synthesis
发明人：ASTATKE MEKBIB; CHATTERJEE DEB K; GERARD GARY F
权利人:LIFE TECHNOLOGIES CORP
摘要:The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot startâ€? synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase acivity.

专利号:US-2002037834-A1
优先权日:2000-09-08
标题 :Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
发明人：ASTATKE MEKBIB; GEBEYEHU GULILAT
权利人:INVITROGEN CORP
摘要:The present invention relates to cationic and polycationic compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses cationic and polycationic molecules, compounds, and compositions having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis. The cationic and polycationic molecules, compounds, and compositions of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot startâ€? synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the cationic and polycationic molecules, compounds, and compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to compositions useful for inhibiting or preventing degradation of various nucleic acid molcules.