CAS: 81691-06-7; 3-Methyl-5-β-D-Ribofuranosyl-2,4(1H,3H)-Pyrimidinedione

上下游产品

1-Acetyl-5-((2S,3S,4R,5R)-3,4-bis-trimethylsilanyloxy-5-trimethylsilanyloxymethyl-tetrahydro-furan-2-yl)-3-methyl-1H-pyrimidine-2,4-dione 3-methyl-3',5'-O-(1,1,3,3-tetraisopropyl-1,3-disiloxanediyl)pseudouridine 3',5'-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)pseudouridine pseudouridine 2'-deoxy-3-methyl-ψ-uridine

📜假尿苷置于吡啶,四甲基乙二胺,氢氟酸,氨,三乙胺体系中,用 二氯甲烷,乙腈,苯 作为反应溶剂,化学反应 102.0H,反应生成 3-甲基-5-Beta-D-呋喃核糖基-2,4(1H,3H)-嘧啶二酮
参考文献:大肠杆菌23S Rrna螺旋69的合成,该螺旋包含其天然修饰的核苷m(3)psi和psi.
标题:大肠杆菌23S Rrna螺旋69的合成,该螺旋包含其天然修饰的核苷m(3)psi和psi.
摘要:3-甲基伪氨吡啶(m(3)psi)亚磷酰胺,5'-O-[苯甲氧基双(三甲基甲硅烷氧基)甲硅烷基]-2'-O-[双(2-乙酰氧基乙氧基)甲基]-3-甲基伪氨吡啶-3'-的合成据报道有(甲基-N,N-二异丙基)亚磷酰胺.对psi N1进行选择性的新戊酰氧基甲基保护,然后在n3处进行甲基化,以反应生成天然存在的伪尿苷类似物.m(3)psi亚磷酰胺与假尿苷(psi)和标准碱基亚磷酰胺结合使用可合成代表大肠杆菌23S核糖体rna(rrna)(残基1906-1924)的螺旋69的19个核苷酸的rna. (3)位置1915处的psi和位置1911和1917处的两个psi.我们对完全修饰的螺旋69 Rna的合成证明了制造毫克量rna的能力,可用于进一步的高分辨率结构研究.于位置1915处假尿苷n3位置的甲基的位点选择性引入导致rna发夹相对于假尿苷的热力学稳定性略有增加;在1915位含有尿苷或3-甲基
DOI:10.1021/jo026364M

专利信息

专利号:US-2014378538-A1
优先权日:2011-12-14
标 题:Methods of responding to a biothreat
发明人：BANCEL STEPHANE
权利人:MODERMA THERAPEUTICS INC
摘要:The present disclosure provides for devices, in particular mobile devices, which may be used in the synthesis of modified nucleic acid molecules, in particular modified mRNA molecules. The device for making the modified nucleosides, modified nucleotides and modified nucleic acids (e.g., mRNA) disclosed herein may be mobile devices comprising at least one sample block for insertion of one or more sample vessels, a device base with electronic control units for the sample block, a voltage supply, and one or more reagent(s) for the synthesis of at least one nucleic acid.

专利号:US-2023220379-A1
优先权日:2021-09-24
标 题 :HIGH PURITY gRNA SYNTHESIS PROCESS
发明人：DAS RAJAT; SANKARAN GANAPATHY
权利人:CRISPR THERAPEUTICS AG
摘要:The present disclosure relates to methods, compositions and kits for synthesizing moderate length RNAs (mlRNAs, including gRNAs) by splint-mediated ligation of RNA fragments. The synthesis of moderate length RNAs can be followed by DNase treatment. In some embodiments, splint DNA oligonucleotides that are no longer than 32 nucleotides are used.

专利号:US-9790531-B2
优先权日:2012-08-14
标 题 :Enzymes and polymerases for the synthesis of RNA
发明人：WANG YUXUN; RAMAKRISHNAN DIVAKAR; DE FOUGEROLLES ANTONIN; WHORISKEY SUSAN
权利人:MODERNATX INC
摘要:The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmRNA molecules.

专利号:US-2025207163-A1
优先权日:2022-03-25
标 题:Methods and apparatus for synthesizing nucleic acids
发明人：HOPKINS PATRYCJA A; SHAHSAVARI SHAHIEN; BEGOYAN VAGARSHAK; NJUMA OLIVE J; DEBENHAM HOLLY S; EFCAVITCH J WILLIAM; ALBIZATI KIM F
权利人:MOLECULAR ASSEMBLIES INC
摘要:Methods for the synthesis of polynucleotides using polymerases nucleotide analogs with labile terminator groups that allow stepwise addition of nucleotides are described. These nucleotide analogs may have a modification on 3′, 2′, or 3′-2′-bridged hydroxyl groups or on nucleobase moieties and can be used in the synthesis of natural or modified polynucleotides suitable for use in biological systems. The labile terminator groups are removed under mild conditions or by enzymes. They are accepted as substrates by polymerases. The synthesis may be carried out on a surface.

专利号:WO-2025101943-A1
优先权日:2023-11-10
标 题:Nucleic acid-guided dna synthesis
发明人：STERNBERG SAMUEL HENRY; TANG STEPHEN; ZHANG DENNIS JAMES; WIEGAND TANNER; THOMAS GEORGE JERRIN; LAMPE GEORGE DAVIS; KANG SHANNON
权利人:UNIV COLUMBIA
摘要:The present disclosure provides systems, compositions, methods, and kits, for guided DNA synthesis and genome engineering. Particularly, the present disclosure provides systems, compositions, methods, and kits comprising a defense-associated reverse transcriptase (DRT), or a nucleic acid encoding thereof, and an engineered target nucleic acid comprising one or more sequence of interest, or a nucleic acid encoding thereof.

专利号:WO-2024200820-A1
优先权日:2023-03-30
标 题:Method of synthesis of targeted lipid nanoparticle and uses thereof