CAS: 7558-63-6; Ammonium (S)-2-Amino-4-Carboxybutanoate

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19285-83-7 56-86-0 6893-26-1

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CAS号56-85-9 L-谷氨酰胺 | CAS号56-86-0 L-谷氨酸

📜L-谷氨酰胺置于l-Glutamate Dehydrogenase体系中,化学反应 0.17H,反应生成 谷氨酸一铵
参考文献:Crystal Structure Of Salt-Tolerant Glutaminase From Micrococcus Luteus K-3 In The Presence And Absence Of Its Product L-Glutamate And Its Activator Tris
标题:Crystal Structure Of Salt-Tolerant Glutaminase From Micrococcus Luteus K-3 In The Presence And Absence Of Its Product L-Glutamate And Its Activator Tris
摘要:Glutaminase From Micrococcus Luteus K‐3 [micrococcus Glutaminase (Mglu); 456 Amino Acid Residues (Aa); 48 Kda] Is A Salt‐tolerant Enzyme. Our Previous Study Determined The Structure Of Its Major 42‐kda Fragment. Here,Using New Crystallization Conditions,We Determined The Structures Of The Intact Enzyme In The Presence And Absence Of Its Product L‐glutamate And Its Activator Tris,Which Activates The Enzyme By Sixfold. With The Exception Of A 'lid' Part (26‐29 Aa) And A Few Other Short Stretches,The Structures Were All Very Similar Over The Entire Polypeptide Chain. However,The Presence Of The Ligands Significantly Reduced The Length Of The Disordered Regions: 41 Aa In The Unliganded Structure (N),21 Aa For L‐glutamate (G),8 Aa For Tris (T) And 6 Aa For Both L‐glutamate And Tris (Tg). L‐glutamate Was Identified In Both The G And Tg Structures,Whereas Tris Was Only Identified In The Tg Structure. Comparison Of The Glutamate‐binding Site Between Mglu And Salt‐labile Glutaminase (Ybgj) From Bacillus Subtilis Showed Significantly Smaller Structural Changes Of The Protein Part In Mglu. A Comparison Of The Substrate‐binding Pocket Of Mglu,Which Is Highly Specific For L‐glutamine,With That Of Erwinia Carotovora Asparaginase,Which Has Substrates Other Than L‐glutamine,Shows That Mglu Has A Larger Substrate‐binding Pocket That Prevents The Binding Of L‐asparagine With Proper Interactions.Structured Digital Abstractmint‐7305730: Mglu (Uniprotkb:Q4U1A6) And Mglu (Uniprotkb:Q4U1A6) Bind (Mi:0407) By X‐ray Crystallography (Mi:0114)
DOI:10.1111/j.1742-4658.2009.07523.X

专利信息

专利号:US-8492115-B2
优先权日:2005-10-31
标 题:Cell-free synthesis of membrane bound polypeptides
发明人：SWARTZ JAMES ROBERT; WUU JESSICA
权利人:SWARTZ JAMES ROBERT; WUU JESSICA; UNIV LELAND STANFORD JUNIOR
摘要:Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane-associated polypeptides.

专利号:US-7871794-B2
优先权日:2007-01-18
标 题:Enhanced cell-free synthesis of active proteins containing disulfide bonds
发明人：KNAPP KURTIS G; SWARTZ JAMES ROBERT
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.

专利号:US-2005054044-A1
优先权日:2003-07-18
标 题:Method of alleviating nucleotide limitations for in vitro protein synthesis
发明人：SWARTZ JAMES ROBERT; JEWETT MICHAEL CHRISTOPHER; CALHOUN KARA ANNE
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the improved in vitro synthesis of polypeptides, where the duration of detectable protein synthesis in a reaction is substantially extended over existing methods, thereby providing for increased total yield of polypeptide. Increased synthesis is accomplished by maintaining the concentration of CTP and UTP in the reaction mixture at a pre-determined level. In another embodiment of the invention, increased synthesis is obtained by maintaining the concentration of cysteine and serine at a pre-determined level. The reaction mixture may be supplemented with additional amino acids during the course of the reaction.

专利号:US-7312049-B2
优先权日:2005-06-14
标题:Total amino acid stabilization during cell-free protein synthesis
发明人：CALHOUN KARA ANNE; SWARTZ JAMES ROBERT
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.

专利号:US-2016115487-A1
优先权日:2013-05-29
标 题 :Cell-free synthetic incorporation of non-natural amino acids into proteins
发明人：BUNDY BRADLEY C; SMITH MARK T; WU JEFFREY C
权利人:BUNDY BRADLEY C; SMITH MARK T; WU JEFFREY C; UNIV BRIGHAM YOUNG
摘要:The present disclosure provides methods for incorporating at least one non-natural amino acid into a polypeptide using a cell-free protein synthesis which includes a cell-free extract and is deficient in endogenous tRNA. The methods include providing the synthesis system with at least one non-natural amino acid, at least one orthogonal tRNA and one orthogonal aminoacyl-tRNA synthetase which aminoacylates the corresponding orthogonal tRNA with the non-natural amino acid. The methods may also include removing the endogenous tRNA in the cell-free protein synthesis system by treating the synthesis system with a ribonuclease to degrade the endogenous tRNA and providing the synthesis system with a minimal set of tRNAs for one or more natural amino acids and the corresponding amino-acyl tRNA synthetases, such that the lack of some tRNA will enable unique codons to encode for non-natural amino acid incorporation with no or a minimal amount of completion from the tRNA present.

专利号:US-9650621-B2
优先权日:2012-10-12
标 题:Proteolytic inactivation of select proteins in bacterial extracts for improved expression
发明人：THANOS CHRISTOPHER D; MURRAY CHRISTOPHER J; YANG JUNHAO; STEPHENSON HEATHER
权利人:SUTRO BIOPHARMA INC
摘要:The present disclosure provides modified proteins that are capable of being cleaved by the protease OmpT1. The proteins can be modified in an exposed surface motif to incorporate OmpT1 cleavage sites. Also provided are nucleic acids encoding the modified proteins, bacterial cells that express the modified proteins, and cell free synthesis systems containing modified RF1. The disclosure further provides methods for reducing the deleterious activity of a modified protein in a cell free synthesis system by contacting the modified protein with OmpT1. Also provided are methods for reducing RF1 competition at an amber codon in the cell free synthesis system, and methods for expressing a protein in the cell free synthesis system. The modified proteins of the invention can be used to increase the yield of proteins having non-natural amino acids incorporated at an amber codon.