CAS: 57-00-1; 2-(1-Methylguanidino)Acetic Acid

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CAS号420-04-2 氰胺 | CAS号4316-73-8 肌氨酸钠 | CAS号156-62-7 氰氨化钙 | CAS号60-27-5 肌酐 | CAS号107-97-1 肌氨酸 | CAS号1758-73-2 二氧化硫脲 | CAS号63-68-3 L-蛋氨酸 | CAS号352-97-6 乙酸胍 | CAS号67-07-2 磷酸肌酸 | CAS号62-49-7 胆碱 | CAS号74-89-5 氨基甲烷 | CAS号25221-53-8 (1-METHYL-4-OXO... | CAS号298-12-4 乙醛酸 | CAS号616-04-6 1-甲基海因 | CAS号76180-96-6 2-氨基-3-甲基-3H-咪唑... | CAS号60-27-5 肌酐 | CAS号15366-32-2 肌酸乙酯盐酸盐 | CAS号141-78-6 乙酸乙酯 | CAS号471-29-4 甲胍 | CAS号144-62-7 草酸

磷酸肌酸置于recombinant Bacillus Subtilis Protein Arginine Phosphatase Ywle体系中,用 Aq. Buffer 作为反应溶剂,化学反应 1.0H,反应生成 肌酸
参考文献:金属阳离子和氧阴离子在枯草芽孢杆菌ywle蛋白精氨酸磷酸酶活性的调节中的作用.
标题:金属阳离子和氧阴离子在枯草芽孢杆菌ywle蛋白精氨酸磷酸酶活性的调节中的作用.
摘要:蛋白精氨酸磷酸化(parg)是一种相对新颖的翻译后修饰.蛋白精氨酸磷酸酶ywle负调节精氨酸磷酸化,因此诱导了应激反应基因的表达,这对于细菌的耐压力和致病性同源金黄色葡萄球菌毒力至关重要.然而,除了氧化应激的影响外,关于影响ywle酶活性的因素知之甚少.本文中,基于ywle水解发色底物对硝基苯基磷酸酯(p Npp),我们研究了金属阳离子和氧阴离子在ywle活性调节中的作用.有趣的是,在我们测试的各种阳离子中,Ca 2+激活ywle,而其他阳离子(包括ag +,Co 2 +,Cd 2+和zn 2+)具有抑制作用.此外,作为磷酸盐的化学类似物,氧阴离子在磷酸酶活性中起多种作用.催化位点内的调节开关cys调节ywle活性.具体地,该cys的硫醇可以被iam(碘乙酰胺)烷基化或被H2O 2氧化,从而导致酶促抑制.相反,还原剂,例如dtt(二硫苏糖醇),β-Me(β-巯基乙醇)和tcep(三(2
DOI:10.1016/j.Bbagen.2020.129698

专利信息

专利号:US-2012190064-A1
优先权日:2004-10-01
标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
发明人：KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA
权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP
摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

专利号:US-6168931-B1
优先权日:1999-03-17
标题 :Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
发明人：SWARTZ JAMES R; KIM DONG-MYUNG
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of biological molecules where ATP is required for synthesis. Of particular interest is the synthesis of polymers, e.g. nucleic acids, polypeptides, and complex carbohydrates. A homeostatic system is used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. Since inorganic phosphate can be an inhibitory by-product of synthesis, the period of time when synthesis is maintained in vitro can be extended. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

专利号:US-6783957-B1
优先权日:1999-03-25
标题:Method for synthesis of polypeptides in cell-free systems
发明人：BIRYUKOV SERGEY VLADIMIROVICH; SIMONENKO PETER NIKOLAEVICH; SHIROKOV VLADIMIR ANATOLIEVICH; SPIRIN ALEXANDER SERGEYEVICH
权利人:ROCHE DIAGNOSTICS GMBH; INST OF PROTEIN RES
摘要:The method of polypeptide synthesis in eukaryotic or porkaryotic cell-free systems based on a modified verion of synthesis in the continuous flow or continuous exchange modes when, in addition to input into the reaction mixture of components maintaining the synthesis and removal from the reaction mixture of low molecular weight components inhibiting the synthesis, the concentration of at least one of the components selected from the group consisting of Mg 2+ , K + , NTP, polyamines or their combinations determining the productivity of the synthesis is continuously changed within the given range of concentrations, while the concentrations of the other components are maintained constant.

专利号:US-2005153390-A1
优先权日:2002-05-14
标 题 :Lyophilized preparation for synthesis of cell-free protein
发明人：ENDO YAETA; OGASAWARA TOMIO
权利人:CELLFREE SCIENCES CO LTD
摘要:Lyophilized preparations from cell extract for use in cell-free protein, which exhibit an activity for protein synthesis being in no way inferior to that exhibited in low-temperature storage; and a method of utilizing the same. In particular, preparations are formed by performing lyophilization after reducing the concentration of deliquescent substances in a solution containing cell extract for synthesis of cell-free protein to a level not detrimental to the quality of preparations after lyophilization.

专利号:US-6994986-B2
优先权日:1999-03-17
标 题:In vitro synthesis of polypeptides by optimizing amino acid metabolism
发明人：SWARTZ JAMES ROBERT; KIM DONG-MYUNG
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides. In order to improve the performance of in vitro protein synthesis reactions, metabolic inhibitors, or manipulation of a source organism, is used to diminish or avoid the action of enzymes responsible for undesirable amino acids production or depletion. A homeostatic system may be used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

专利号:EP-1525305-B1
优先权日:2002-08-02
标题:Methods and compositions for in vitro synthesis of proteins in a cell-free system enriched with atp-sulfurylase
发明人：RYABOVA LYUBOV; MASSON JEAN-MICHEL
权利人:PROTEUS
摘要:The present invention provides methods and composition for enhancing in vitro synthesis of biological macromolecules in a cell-free system where ATP is required as a primary energy source, wherein said cell-free system is enriched with ATP-sulfurylase The invention relates also to as cell-free systems and cell-free extracts enriched with ATP-sulfurylase for enhancing in vitro synthesis of any biological macromolecules.