1-octadecylmagnesium Bromide置于四氯化硅,乙醚,苯体系中,化学反应生成十八烷基三氯硅烷 参考文献:Alkyl Silicon Chlorides And Their Preparation 标题:Alkyl Silicon Chlorides And Their Preparation
专利号:US-2023313255-A1 优先权日:2020-07-15 标题:Massively Parallel Enzymatic Synthesis of Polynucleotides 发明人:HORGAN ADRIAN; LACHAIZE HENRI; VERARDO DOMIANO; GODRON XAVIER 权利人:DNA SCRIPT 摘要:The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.
专利号:US-2024024489-A1 优先权日:2020-11-20 标 题:Protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof 发明人:MULARD LAURENCE; DHARA DEBASHIS; PFISTER HELENE; PAOLETTI JULIE; PHALIPON ARMELLE; GUERREIRO-INVERNO CATHERINE 权利人:PASTEUR INSTITUT 摘要:The present invention provides zwitterionic oligosaccharides, in particular fragments of the surface polysaccharides from Shigella sonnei and Shigella sonnei conjugates comprising them. The present invention also provides protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof, the disaccharide repeating unit of Shigella sonnei being: (I)
专利号:US-2023323449-A1 优先权日:2021-12-17 标 题:Compositions and methods for detection of variants 发明人:GANTT RICHARD; ARNESON ADRIANA 权利人:TWIST BIOSCIENCE CORP 摘要:Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of ‘construction’ oligonucleotides and tagged complementary ‘selection’ oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.
专利号:US-9981239-B2 优先权日:2015-04-21 标题:Devices and methods for oligonucleic acid library synthesis 发明人:BANYAI WILLIAM; PECK BILL JAMES; FERNANDEZ ANDRES; CHEN SIYUAN; INDERMUHLE PIERRE; MARSH EUGENE P 权利人:TWIST BIOSCIENCE CORP 摘要:Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
专利号:WO-2024153643-A1 优先权日:2023-01-16 标 题 :Inkjet-assisted enzymatic nucleic acid synthesis 发明人:MOGHADDAM NICOLAS; ADELIZZI BEATRICE; LOMAN TESSA; VERARDO DAMIANO; RODRIGUEZ-PINZON DANIEL; HORGAN ADRIAN 权利人:DNA SCRIPT 摘要:Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides.
专利号:US-11512347-B2 优先权日:2015-09-22 标 题 :Flexible substrates for nucleic acid synthesis 发明人:PECK BILL JAMES 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Additionally, devices described herein for de novo synthesis of oligonucleic acids encoding information related to the original source information may have a flexible material for oligonucleic acids extension.