专利号:US-9995750-B2 优先权日:2013-12-05 标 题:Methods of detecting and measuring glutamine and analogues thereof, and methods related thereto 发明人:ACKERLEY DAVID FRANCIS; BROWN ALISTAIR SINCLAIR; ROBINS KATHERINE JANE 权利人:VICTORIA LINK LTD 摘要:Method for the detection of glutamine and its analogs are provided by the present disclosure. Also provided are methods for measuring the levels of glutamine and its analogs, including diagnostic methods. Further provided are methods that utilize glutamine analogs for the synthesis of colored pigments and other useful agents. The methods comprise the use of a nonribosomal peptide synthetase (NRPS) under conditions to produce an indigoidine or indigoidine-related pigment.
专利号:US-2016238611-A1 优先权日:2013-10-02 标题:Non-ribosomal protein synthesis pigment fusion peptides 发明人:EILS ROLAND; DI VENTURA BARBARA; ADLUNG LORENZ; GENREITH KATHARINA; HEINEMANN TIM; MEYER HANNAH; NIOPEK DOMINIK; GEORGI FANNY; BEER RALF; CHRISTIANSEN TANIA CHRISTINA; HERBST KONRAD; IGNATIADIS NIKOLAOS; KATS ILIA; KURZAWA NILS; MEICHSNER JOHANNA; RABE SOPHIE; RIEDEL ANJA; SACHS JOSHUA; SCHESSNER JULIA PATRICIA; SCHMIDT FLORIAN; WALCH PHILIPP DARIUS KONSTANTIN 权利人:DEUTSCHES KREBSFORSCH; Ruprecht-Karls-Universität Heidelberg 摘要:The present invention relates to a polypeptide or polypeptide complex, comprising at least one non-ribosomal peptide synthesis (NRPS) amino acid module functionally connected to at least one pigment module. The present invention further relates to a labeled oligopeptide comprising a non-naturally attached NRPS pigment or/and polyketide pigment, to a polynucleotide encoding a fusion polypeptide, a vector, preferably an expression vector, comprising the polynucleotide of the present invention and to a host cell comprising the polypeptide or polypeptide complex and/or the polynucleotide, and/or the vector according to the present invention. Moreover, the present invention relates to in vitro and in vivo method of producing a labeled oligopeptide, as well as to methods of optimizing the same.
专利号:JP-7194741-B2 优先权日:2018-01-31 标题:Genetic recombination method to catalyze the synthesis of glutamine to indigoidin to obtain blue flowers
专利号:JP-2021511810-A 优先权日:2018-01-31 标题:Genetically modified method to catalyze the synthesis of glutamine to indigoidin to obtain blue flowers
专利号:CN-108330146-A 优先权日:2018-01-31 标题:A transgenic method for obtaining blue flowers by catalyzing the synthesis of indigo from glutamine
专利号:CN-118126914-A 优先权日:2023-12-25 标题:Recombinant Corynebacterium glutamicum and its application in fermentation synthesis of microbial indigo pigment and method for quantitative analysis of L-glutamine
参考文献:10.1038/srep41745 摘要:Brown AS, Robins KJ, Ackerley DF. A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples. Scientific Reports. 2017 Jan 31;7(1):41745. doi: 10.1038/srep41745.