专利号:US-2024391961-A1 优先权日:2021-08-25 标题:Autonomous organisms for synthesis of permanently phosphorylated proteins 发明人:COOLEY RICHARD B; MEHL RYAN A; ZHU PHILLIP 权利人:UNIV OREGON STATE 摘要:Embodiments of the present disclosure provide compositions and methods for biosynthesizing a stable, functional mimic of phosphoserine. The compositions and methods described herein genetically program a host cell to express a biosynthetic pathway that can synthesize the amino acid, 2-amino-4-phosphobobutyric acid, that is a non-hydrolyzable phosphoserine (nhpSer) because it contains a carbon-phosphorus, e.g., phosphonate, bond. In one embodiment the genetically programed cells express the enzymes of a pathway from a Streptomyces bacterium . In some embodiments, nhpSer is translationally incorporated into protein of interest at one or more programmed UAG amber codons using Genetic Code Expansion (GCE) technology.
专利号:US-9995750-B2 优先权日:2013-12-05 标 题:Methods of detecting and measuring glutamine and analogues thereof, and methods related thereto 发明人:ACKERLEY DAVID FRANCIS; BROWN ALISTAIR SINCLAIR; ROBINS KATHERINE JANE 权利人:VICTORIA LINK LTD 摘要:Method for the detection of glutamine and its analogs are provided by the present disclosure. Also provided are methods for measuring the levels of glutamine and its analogs, including diagnostic methods. Further provided are methods that utilize glutamine analogs for the synthesis of colored pigments and other useful agents. The methods comprise the use of a nonribosomal peptide synthetase (NRPS) under conditions to produce an indigoidine or indigoidine-related pigment.
专利号:WO-2020237701-A1 优先权日:2019-05-30 标题:High-yield l-histidine genetically engineered bacterium strain, and construction method therefor and application thereof 发明人:XIE XIXIAN; ZHANG YUE; FAN WEIMING; JIANG WEI; WU HEYUN; CHEN YANNA; TIAN DAOGUANG; TU JIANQING 权利人:TIANJIN UNIV OF SCIENCE&TECHNOLOGY; ZHEJIANG ZHENYUAN PHARMACEUTICAL CO LTD 摘要:Provided are a high-yield L-histidine genetically engineered bacterium strain, and a construction method therefor and an application thereof, integrating a corynebacterium glutamicum ATP phosphoribosyl transferase HisG mutant encoding gene into a genome of escherichia coli to enhance the activity of HisG, also increasing the number of copies of histidine operon genes in the genome to enhance synthesis of histidine, and integrating an encoding gene lysE of arginine/lysine transport protein derived from the corynebacterium glutamicum into the genome to promote extracellular secretion of intracellular histidine. The genetically engineered bacterium is used for producing the L-histidine by a fermentation method, can stably produce 40-55 g/L of the histidine by fermentation in a 5 L fermentation tank for 40-50 h, has a production intensity reaching 1.0-1.5 g/(Lxh), and has a conversion rate of 0.18-0.22 g histidine/g glucose.
专利号:US-9439423-B2 优先权日:2007-10-29 标 题 :Antiparasitic agent for fish and method of controlling proliferation of fish parasites 发明人:KAWANO FUMI; HIRAZAWA NORITAKA 权利人:KAWANO FUMI; HIRAZAWA NORITAKA; NIPPON SUISAN KAISHA LTD 摘要:An antiparasitic agent for fish includes an inhibitor of folate synthesis and/or an inhibitor of folate activation as the active substance(s). A combination preparation composed of an inhibitor of folate synthesis and an inhibitor of folate activation is disclosed. Sulfonamide is disclosed as suitable for the inhibitor of folate synthesis. A dihydrofolate reductase inhibitor, a folate antagonist are disclosed as suitable inhibitors of folate activation. The antiparasitic agent is able to exterminate fish parasites via oral administration. It is effective against fish parasites belonging to the ciliate group.
专利号:US-6858434-B1 优先权日:1999-10-06 标 题:Method for synthesis, separation and screening of a plurality of compounds in the same bulk of a stationary phase 发明人:WILLIAMS LORENZO 权利人:SINVENT AS 摘要:A method for sequentially performing a synthesis, separation and screening of chemical entities, especially a combinatorial library, is described. The method utilises a bulk of a stationary phase (e.g. silica gel, aluminium oxide, cellulose, etc. for example arranged on a backing) for the performance of the synthesis, separation and screening. The technique described enables a rapid route from synthesis to the testing of chemical compounds. Screening can be performed without need for reaction work-up. Preferred screening methods are those used to determine the biological activity of the compounds.
专利号:US-9265777-B2 优先权日:2009-04-27 标题 :Antiparasitic agent for fish and method of controlling proliferation of fish parasites 发明人:KAWANO FUMI; HIRAZAWA NORITAKA 权利人:KAWANO FUMI; HIRAZAWA NORITAKA; NIPPON SUISAN KAISHA LTD 摘要:A method of controlling proliferation of fish parasites comprising the administration of 1 to 50 mg/kg fish body weight/day of an inhibitor of folate synthesis and/or an inhibitor of folate activation to fish continuously for 1 to 2 weeks. Using a combination preparation composed of an inhibitor of folate synthesis and an inhibitor of folate activation is preferable, and a sulfonamide is preferable for the inhibitor of folate synthesis. A dihydrofolate reductase inhibitor, a folate antagonist, etc., can be used as the inhibitor of folate activation. The antiparasitic agent is able to exterminate fish parasites via oral administration. It is particularly effective against parasites belonging to the ciliate group among fish parasites.
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