S-1401

Chemical Name: 5'-O-[3,4-Bis(benzyloxy)benzyl]thymidylyl-(3'-5')-2'-deoxyguanylyl-(3'-5')-2'-deoxyguanylyl-(3'-5')-2'-deoxyguanylyl-(3'-5')-2'-deoxyadenylyl-(3'-5')-2'-deoxyguanosine penta(N,N-diethylethanamine) inner salt
CAS No. 185041-17-2, 167146-19-2 (free base)
项目整合开发状态: Biological Testing
项目研究机构: Sankyo (Originator)
合成路线:5'-O-(3,4-dibenzyloxybenzyl)thymidine (I) was converted to the corresponding phosphoramidite (III) upon treatment with 2-cyanoethyl-N,N-diisopropylchlorophosphoramidite (II) in the presence of diisopropylethylamine.This was applied to an automatic DNA synthesizer and coupled to the polymer support-linked pentadeoxyribonucleotide sequence GGGAG.The resulting oligonucleotide was liberated from the solid support using aqueous ammonia,and then purified by reverse phase-HPLC in the presence of a triethylammonium acetate buffer to furnish the title compound as the triethylammonium salt.