新产品编号:1335717

Chemical Name: 6-Carbamoyl-4(S)-[5(S)-(cyclopentylsulfanylcarboxamido)-2(R)-(4-fluorobenzyl)-6-methyl-4-oxoheptanoyl]-2(E)-hexenoic acid ethyl ester
项目整合开发状态: Biological Testing
项目研究机构: Agouron (Originator)
合成路线:Tetrahydrofuranone (XIII): The Grignard condensation of isobutyraldehyde (I) with vinylmagnesium bromide (II) in THF gives the magnesium alcoholate (III),which is condensed with ethyl malonyl chloride (IV) in the same solvent,yielding the mixed ester (V).Treatment of (V) with Ti(OEt)4 at 190 C affords 6-methyl-4(E)-heptenoic acid methyl ester (VI),which by reaction with (R,R)-(-)-pseudoephedrine (VII),oxalyl chloride and DMF in benzene gives the amide (VIII).The regiocontrolled addition of 4-fluorobenzyl chloride (IX) to the chiral amide (VIII) by means of BuLi and LiCl in THF yields the 2(S)-(4-fluorobenzyl)heptanamide (X),which by reaction with NBS in THF/water/acetic acid at 0 C,followed by reflux for 45 min,affords 5(S)-[1(R)-bromo-2-methylpropyl]-3(R)-(4-fluorobenzyl)tetrahydrofuran-2-one (XI).The reaction of (XI) with NaN3 in DMF gives the corresponding azide (XII),which is reduced with H2 over Pd/C,and the resulting amine is protected with tert-butoxycarbonyl anhydride to obtain the desired tetrahydrofuranone (XIII).

合成路线:Final coupling: Deprotection of (XXVI) with HCl in dioxane followed by condensation with tetrahydrofuranone (XIII) by means of DIEA in DMF gives the amide (XXVII).This compound is also deprotected with HCl as before and condensed with 5-methylisoxazol-3-ylcarbonyl chloride (XVIII) by means of pyridine to afford the protected compound (XXIX),which is finally treated with DDQ,yielding AG-7088.

合成路线:Isobutyraldehyde (I) was condensed with vinylmagnesium bromide (II) to provide allylic alcohol (III).Condensation of (III) with diethyl malonate (IV) in the presence of titanium ethoxide,followed by Claisen rearrangement at 190 C gave malonate (V).Subsequent basic hydrolysis of (V) with concomitant decarboxylation yielded 6-methyl-4-heptenoic acid (VI).This was transformed to the corresponding acid chloride by means of SOCl2 and then coupled with (1R,2R)-(-)-pseudoephedrine (VII) to produce the chiral amide (VIII).Alkylation of the dianion of (VIII) with benzyl bromide (IX) in the presence of LiCl afforded the benzylated compound (X).Further treatment of (X) with N-bromosuccinimide and AcOH in THF generated the bromolactone (XI).The bromo group of (XI) was then displaced with NaN3,and the resulting azide (XII) was hydrogenated in the presence of di-tert-butyl dicarbonate to provide carbamate (XIII).Basic hydrolysis of the lactone (XII) gave hydroxyacid (XIV),which was oxidized to ketoacid (XV) by means of N-methylmorpholine-N-oxide and tetrapropyl ammonium perruthenate.

合成路线:Acid (XV) was coupled with protected amino acid (XVI) to give amide (XVII),and the Boc protecting group of (XVII) was then removed under acidic conditions.The resulting amine (XVIII) was condensed with cyclopentyl chlorothioformate (XIX) to provide thiocarbamate (XX).Finally,the title compound was obtained by trifluoroacetic acid-promoted cleavage of the trityl protecting group of (XIX).

合成路线:Isobutyraldehyde (I) was condensed with vinylmagnesium bromide (II) to provide allylic alcohol (III).Transesterification with diethyl malonate (IV) in the presence of titanium ethoxide,followed by Claisen rearrangement at 190 C gave malonate (V).Subsequent basic hydrolysis of (V) with concomitant decarboxylation yielded 6-methyl-4-heptenoic acid (VI).This was transformed to the corresponding acid chloride by means of SOCl2 and then coupled with (1R,2R)-(-)-pseudoephedrine (VII) to produce the chiral amide (VIII).Alkylation of the dianion of (VIII) with 4-methylbenzyl bromide (IX) in the presence of LiCl afforded the benzylated compound (X).Further treatment of (X) with N-bromosuccinimide and AcOH in THF generated the bromolactone (XI).The bromo group of (XI) was then displaced with NaN3,and the resulting azide (XII) was hydrogenated in the presence of di-tert-butyl dicarbonate to provide carbamate (XIII).Basic hydrolysis of the lactone (XIII) gave hydroxyacid (XIV),which was oxidized to ketoacid (XV) by means of N-methylmorpholine-N-oxide and tetrapropyl ammonium perruthenate.

合成路线:Acid (XV) was coupled with protected amino acid (XVI) to give amide (XVII),and the Boc protecting group of (XVII) was then removed under acidic conditions.The resulting amine (XVIII) was condensed with cyclopentyl chlorothioformate (XIX) to provide thiocarbamate (XX).Finally,the title compound was obtained by trifluoroacetic acid-promoted cleavage of the trityl protecting group of (XIX).