NNC-63-0532

甲基[8-(2-萘基甲基)-4-氧代-1-苯基-1,3,8-三氮杂螺[4.5]癸-3-基]乙酸酯Chemical Name: 2-[8-(1-Naphthylmethyl)-4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]dec-3-yl]acetic acid methyl ester
CAS No. 250685-44-0
项目整合开发状态: Preclinical
项目研究机构: Novo Nordisk (Originator)
合成路线:Solid phase synthesis: Attachment of 8-Fmoc-3-carboxymethyl-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one (I) to Wang resin by means of 2,6-dichlorobenzoyl chloride (A) in DMF in the presence of pyridine,followed by treatment with benzoyl chloride (B) in dichloromethane in the presence of pyridine,provides resin-anchored derivative (II).Treatment of resin (II) with piperidine in DMF allows Fmoc removal to yield deprotected piperidine derivative (III),which is then converted into the target compound by alkylation with 1-(bromomethyl)naphthalene (IV) by heating in DMSO in the presence of DIEA,followed by cleavage of the molecule from the resin by heating with NaOMe in THF/MeOH.Alternatively,the conversion of (III) into the desired compound can be achieved by reductive amination between resin (III) and aldehyde (V) by means of NaBH3CN in THF in the presence of HOAc to afford anchored derivative (VI),followed by cleavage from the resin by heating with NaOMe in THF/MeOH.

合成路线:Solution synthesis: In this case the target product is obtained by alkylation of 1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one (VII) with 1-(chloromethyl)naphthalene (VIII) by means of K2CO3 and NaI in refluxing 2-butanone,followed by acylation with methyl bromoacetate (X) in DMF by means of NaH.