Allicin
大蒜素Chemical Name: 2-Propene-1-sulfinothioic acid S-2-propenyl ester; Diallyl disulfide-oxide; 2-Propenethiosulfinic acid S-2-propenyl ester; Allylthiosulfinic acid S-allyl ester
CAS No. 539-86-6
项目整合开发状态: Phase II
项目研究机构: Tulane University (Originator), Weizmann Institute of Science (Originator)
合成路线:Allicin was prepared daily for our animal studies by reacting alliin and alliinase.Alliin was produced by a modified method developed by Stoll and Seebeck.The chemical and spectral properties of the synthetic alliin were identical to those of the natural product in the garlic clove.Alliinase extraction and purification were performed as described previously.The enzymatic activity of alliinase and the rate of production of allicin and pyruvate were determined by spectrophotometry in a coupled reaction which uses reduced nicotinamide adenine dinucleotide and lactic dehydrogenase to generate nicotinamide dinucleotide and lactic acid.The allicin was prepared just before administration by interaction of alliin (10 mg/kg rabbit) with purified alliinase (3 U/mg alliin).One unit of alliinase is defined as the amount of enzyme needed to produce 1 mcmol of pyruvate from alliin per minute.The amount of allicin produced was confirmed by a high-performance liquid chromatography analysis of a sample.
📌 参考资料/链接：
参考文献标题:Allicin
文献作者:Eilat,S.; Avramovitch,D.; Rabinkov,A.; Mirelman,A.; Battler,A.; Eldar,M.; Vered,Z.
参考来源:Drugs Fut 1996,21(11),1101
📄 详细内容
合成路线:Allicin was prepared daily for our animal studies by reacting alliin and alliinase.Alliin was produced by a modified method developed by Stoll and Seebeck.The chemical and spectral properties of the synthetic alliin were identical to those of the natural product in the garlic clove.Alliinase extraction and purification were performed as described previously.The enzymatic activity of alliinase and the rate of production of allicin and pyruvate were determined by spectrophotometry in a coupled reaction which uses reduced nicotinamide adenine dinucleotide and lactic dehydrogenase to generate nicotinamide dinucleotide and lactic acid.The allicin was prepared just before administration by interaction of alliin (10 mg/kg rabbit) with purified alliinase (3 U/mg alliin).One unit of alliinase is defined as the amount of enzyme needed to produce 1 mcmol of pyruvate from alliin per minute.The amount of allicin produced was confirmed by a high-performance liquid chromatography analysis of a sample.
参考文献标题:Alliin lyase (allinase) from garlic (Allium sativum): Biochemical characterization and cDNA cloning
文献作者:Rabinkov,A.; Xiao-Zho,Z.; Grafi,G.; Galili,G.; Mirelman,D.
参考来源:Appl Biochem Biotechnol 1994,48(3),149-71
合成路线:Allicin was prepared daily for our animal studies by reacting alliin and alliinase.Alliin was produced by a modified method developed by Stoll and Seebeck.The chemical and spectral properties of the synthetic alliin were identical to those of the natural product in the garlic clove.Alliinase extraction and purification were performed as described previously.The enzymatic activity of alliinase and the rate of production of allicin and pyruvate were determined by spectrophotometry in a coupled reaction which uses reduced nicotinamide adenine dinucleotide and lactic dehydrogenase to generate nicotinamide dinucleotide and lactic acid.The allicin was prepared just before administration by interaction of alliin (10 mg/kg rabbit) with purified alliinase (3 U/mg alliin).One unit of alliinase is defined as the amount of enzyme needed to produce 1 mcmol of pyruvate from alliin per minute.The amount of allicin produced was confirmed by a high-performance liquid chromatography analysis of a sample.
参考文献标题:Chemical investigations of alliin,the specific principle of garlic
文献作者:Stoll,A.; Seebeck,E.
参考来源:Adv Enzymol 1951,11377-400
产品链接: CAS No. 539-86-6››